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A kind of activated T lymphocyte culture fluid and the preparation method of activated T lymphocyte

A lymphocyte and cell culture technology, applied in the direction of cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve the problems of low purity and low efficiency

Active Publication Date: 2021-04-06
蓝莲(杭州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing preparation of activated T lymphocytes generally has problems such as low efficiency and low purity

Method used

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  • A kind of activated T lymphocyte culture fluid and the preparation method of activated T lymphocyte

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Experimental program
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Embodiment 1

[0045] AT training steps:

[0046] 1. Confirm that the suction tube of the plasma bag is sealed. Wipe the pipette and scissors with two different cleanroom wipes soaked in 70% ethanol.

[0047] 2. Hold the suction tube with tweezers, cut the front end of the tube and control the flow rate with tweezers to transfer the plasma into a 50ml test tube. Record the plasma volume transferred.

[0048] 3. Centrifuge the tube (400g, 4°C) for 30 minutes to remove platelets.

[0049] 4. Pour the supernatant liquid into a new 50ml test tube.

[0050] 5. Put the test tube into a constant temperature water tank (56°C) and incubate for 30 minutes.

[0051] 6. Centrifuge tubes (400g, 4°C) for 60 minutes to remove precipitated fibrin.

[0052] 7. Pour the upper liquid (autologous serum) into a new 50ml test tube. Label the tube with the patient's name, date of preparation, and refrigerate (4°C).

[0053] 8. Confirm that the suction tube of the PBMC bag is sealed. Hang the PBMC bags. Wi...

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Abstract

The invention discloses an activated T lymphocyte culture medium and a preparation method of the activated T lymphocyte, belonging to the technical field of cell culture. Including the cultivation of AT cells and harvesting of AT cells. The preparation method of the above-mentioned activated T lymphocyte culture fluid and activated T lymphocytes, which uses Lymactin-T anti-CD3 monoclonal antibody to cooperate with IL-2 and IL-6, can activate T lymphocytes with high efficiency. Cells proliferate rapidly during the induction period; and use the method of PMA and ionomycin to activate T cells; this method has the advantages of high efficiency and high purity.

Description

technical field [0001] The invention belongs to the technical field of cell culture, in particular to an activated T lymphocyte culture medium and a preparation method of the activated T lymphocyte. Background technique [0002] T lymphocytes are derived from pluripotent stem cells of bone marrow. T cell activation technology is to extract blood from the patient, expand and activate it in the laboratory, and finally return these high-number and ultra-highly active T cells back to the patient. , activate the body's humoral and cellular immune systems. The existing preparation of activated T lymphocytes generally has problems such as low efficiency and low purity. Contents of the invention [0003] In view of the above-mentioned problems existing in the prior art, the object of the present invention is to design and provide a kind of activated T lymphocyte culture fluid and the technical scheme of the preparation method of activated T lymphocytes, it uses Lymactin-T anti-CD...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2501/2302C12N2501/2306C12N2501/515C12N2501/999
Inventor 杨罕闻
Owner 蓝莲(杭州)生物科技有限公司