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A bidirectional optical field in situ observation method for marine organisms adapting to targets with different transmittances

A technique adapted to different, marine organisms, applied in the field of optical microscopy, which can solve problems such as difficult to obtain surface details

Active Publication Date: 2020-06-02
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for non-transparent targets with low transmittance, such as copepods and tunicates, they can only be illuminated by front light and imaged by the reflected light of the target. If only the edge image of silhouette effect can be obtained by using back light, it is difficult get surface details
[0004] In the ocean, plankton is the object to be observed, and its appearance is random. The distribution mode of the illumination light field designed by the existing microscopic detection system is unidirectional, and a single incident illumination mode cannot be used in the same system. Under the condition of achieving both transparent and non-transparent target observation

Method used

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  • A bidirectional optical field in situ observation method for marine organisms adapting to targets with different transmittances
  • A bidirectional optical field in situ observation method for marine organisms adapting to targets with different transmittances
  • A bidirectional optical field in situ observation method for marine organisms adapting to targets with different transmittances

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Embodiment 1

[0025] Embodiment 1, refer to image 3 and Figure 5 , image 3 It is a schematic diagram of the principle of the front light illumination light field of the general microscope imaging system, Figure 5 It is a schematic diagram of the plankton microscopic observation area of ​​the present invention. In order to construct a two-way optical field in situ observation method for marine organisms that is suitable for targets with different transmittances. First, choose the general-purpose front light incident illumination mode of the microscopic imaging system in the existing technology, and establish a microscopic incident illumination light field for non-transparent targets. In this light field, the system can use the characteristics of strong reflected light of this type of target, Obtain high signal-to-noise ratio images.

[0026] Figure 5 In the above, we use the common front light incident illumination method of the microscopic imaging system to establish a microscopic...

Embodiment 2

[0027] Embodiment 2, refer to figure 2 , Figure 4 and Figure 5 , figure 2 It is a schematic diagram of the optical principle of the corner cube array of the present invention, Figure 4 A schematic diagram of the two-way illumination light field distribution generated by a corner cube prism array for the present invention, Figure 5 It is a schematic diagram of the plankton microscopic observation area of ​​the present invention.

[0028] refer to figure 2 , The corner cube 1 is a glass element used for retroreflection, and it uses three angles of 90° to retroreflect the incident light beam, and the corner cube 1 adopts a corner cube array. In the same optical path, when the incident illumination is applied to a transparent target, more than 90% of the light energy will pass through the target and cannot participate in imaging. Therefore, if you only rely on reflected light imaging, you cannot obtain clear imaging. The present invention intends to use the lighting p...

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Abstract

Provided in the invention is an in-situ bidirectional light field observation method of a marine organism for different transmittance targets. An objective lens, a light source and a pyramid prism carrier are arranged in water having to-be-observed plankton. A pyramid prism array formed by a plurality of pyramid prisms is arranged on the end surface, facing the objective lens, of the pyramid prismcarrier; and the central axis of the objective lens is perpendicular to the end surface of the pyramid prism carrier. The light source and the objective lens are arranged at the same side. The lightsource is turned on; and a camera carries out shooting. According to the invention, the pyramid prisms are arranged at positions facing the objective lens of the microscope. Since incident light is returned by the pyramid prisms based on original light paths to form retro-reflective light, light rays that are emitted by the same group of incident illuminating sources and pass through transparent targets pass through the pyramid prism array to form retro-reflective light by means of irradiation and illumination before a microscopic observation area, so that the incident light and retro-reflective light coexist and thus a bidirectional illumination light field is formed in the same microscopic observation area. Therefore, transparent and non-transparent targets can be observed simultaneously.

Description

technical field [0001] The invention relates to the field of optical microscopy technology, in particular to a method for forming a two-way optical field in-situ observation method for marine organisms adapting to targets with different transmittances. Background technique [0002] At present, optical imaging technology is an important technical means for in-situ observation of small plankton in the ocean. Among them, ordinary optical imaging can obtain in-situ color optical images of plankton, which is the closest to the currently recognized and reliable laboratory manual microscope observation, and With the advantages of automaticity, high efficiency, and no disturbance, it has been widely used in marine surveys. [0003] In the existing technology, although ordinary optical imaging can obtain the "real image" of the target for real in-situ observation, it cannot solve the problem of the one-way illumination light field under the single incident illumination mode and the o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N21/01
CPCG01N21/01G01N21/84
Inventor 郑冰徐儒于佳付民顾肇瑞
Owner OCEAN UNIV OF CHINA