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A detection method, the technology of acrylamide, applied in the field of detection and analysis, can solve the problems of unqualified detection limit, long detection time, poor reproducibility, etc., and achieve the effect of small standard error, low detection limit and high measurement accuracy
Active Publication Date: 2019-09-06
AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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[0005] For the current detection and analysis method, there are still many defects, such as long detection time, detection limit does not meet the requirements, poor reproducibility, serious impurity interference, etc.
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Embodiment 1
[0031] In this embodiment, fried dough sticks are selected for detection, and the processing method is as follows:
[0032] S1, coarsely pulverize the deep-fried dough sticks to 5 mesh;
[0033] S2, weigh 2g (accurate to 0.001) deep-fried dough stick sample, place it in an environment of 10-15°C for 45s, and then ultrasonically treat it for 60s, with ultrasonic power of 200-500W and ultrasonic frequency of 25-35kHz;
[0034] S3, take the sample treated by S2, put it into the sample container, and add n-hexane solution, the amount of n-hexane solution is 10 times the sample volume (volume mass ratio), and add 10mg / L 13 C 3 20 μL of acrylamide internal standard working solution, mixed thoroughly for degreasing treatment, discarded the n-hexane phase, obtained the sample to be extracted, and blown dry with nitrogen;
[0035] S4, take 30% sodium chloride solution and 10% sodium acetate solution according to the volume ratio of 8:1, mix to obtain the extract, and extract under ce...
Embodiment 2
[0039] In this embodiment, potato chips are selected for detection, and the processing method is as follows: S1, coarsely crush the fried dough sticks, and crush them to 5 mesh;
[0040] S2. Weigh 2g (accurate to 0.001g) fried dough stick sample, place it in an environment of 10-15°C for 30s, and then ultrasonically treat it for 75s. The ultrasonic power is 250-500W and the ultrasonic frequency is 25-30kHz;
[0041] S3, take the sample treated by S2, put it into the sample container, and add n-hexane solution, the amount of n-hexane solution is 8 times the sample volume (volume mass ratio), and add 10mg / L 13 C 3 20 μL of acrylamide internal standard working solution, mixed thoroughly for degreasing treatment, discarded the n-hexane phase, obtained the sample to be extracted, and blown dry with nitrogen;
[0042]S4, take 30% sodium chloride solution and 10% sodium acetate solution according to the volume ratio of 8:1, mix to obtain the extract, and extract under certain temper...
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Abstract
The invention discloses a quick detection method of acrylamide. The method comprises the following steps: coarsely grinding and ultrasonically treating the sample; adding a n-hexane solution in the treated sample, performing ungrease treatment, abandoning n-hexane phase to obtain a to-be-extracted sample; taking a sodium chloride solution and a sodium acetate solution to perform extraction; performing purification treatment, adding carrez reagent I and carrez reagent II in the treated sample, standing, centrifuging, taking supernatant to pass through a SPE column, collecting the to-be-detectedsample solution obtained after filtering; concentrating effluent to nearly dry under the nitrogen flow; and analyzing by using liquid chromatography-mass spectrum / mass spectrometer. The quick detection method disclosed by the invention is high in detection accuracy, and can further promote the acrylamide to dissolve in the extraction solvent when the emulsification phenomenon is eliminated; the effect for removing particles, oils and like impurities are good, the blocking in an extraction column is avoided, the detection limit is low, the determination precision is high, and the acrylamide amount in the sample can be more precisely determined.
Description
technical field [0001] The invention belongs to the technical field of detection and analysis, and in particular relates to a rapid detection method for acrylamide. Background technique [0002] For foods processed at high temperatures such as baking or frying, a hazardous substance, namely acrylamide, is easily formed. Acrylamide was first discovered by Stockholm University in 2002, and the amount of acrylamide contained in fried potatoes exceeded 500 times the operating content in drinking water standards, and the maximum allowable limit in drinking water is 0.0005mg / L. [0003] The molecular weight of acrylamide is 70.08, and it is easily soluble in water, methanol, ethanol, etc. Although the human body can absorb acrylamide through various channels such as the respiratory tract, digestive tract, skin and mucous membranes, the absorption of acrylamide is the fastest through the digestive tract. In fact, acrylamide is not only found in high-temperature processed foods, bu...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062
Inventor 曾绍东杨春亮叶剑芝苏子鹏林玲
Owner AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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