Novel application of bionectria ochroleuca and method for preventing and treating mosquito vector

A new-purpose technology of Pleurotus chinensis, applied in the field of biological control, can solve the problems of vector mosquitoes that have not been reported, and achieve the effect of enhancing mosquito control effect, good mosquito control effect, and reducing resistance and distance

Inactive Publication Date: 2019-09-10
SHANDONG INST OF PARASITIC DISEASES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its use in the control of vector mosquitoes has not been reported

Method used

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  • Novel application of bionectria ochroleuca and method for preventing and treating mosquito vector
  • Novel application of bionectria ochroleuca and method for preventing and treating mosquito vector
  • Novel application of bionectria ochroleuca and method for preventing and treating mosquito vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A method for preventing and controlling vector mosquitoes by Chitosan leucoides, comprising the following steps:

[0045] 1) using the potato dextrose agar medium to cultivate the leucophyte, the incubation temperature is 22 °C, to obtain the leucophyte spores;

[0046] 2) collecting the spores of Trichoderma paleochromatum obtained in step 1) and suspending them in Tween water to prepare a spore suspension;

[0047] 3) The spore suspension is added to the water body containing mosquitoes, that is, the control of vector mosquitoes is completed.

Embodiment 2

[0049] A method for preventing and controlling vector mosquitoes by Chitosan leucoides, comprising the following steps:

[0050] 1) using the potato dextrose agar medium to cultivate the leucophyte, the incubation temperature is 22 °C, to obtain the leucophyte spores;

[0051] 2) mixing the spores of Chitosan lividans obtained in the collection step 1) with diatomite powder to prepare powdery spore powder;

[0052] 3) Adding the powdered spore powder to the water body containing mosquitoes, that is, the control of vector mosquitoes is completed.

Embodiment 3

[0054] A method for preventing and controlling vector mosquitoes by Chitosan leucoides, comprising the following steps:

[0055] 1) using the potato dextrose agar medium to cultivate the leucophyte, the incubation temperature is 22 °C, to obtain the leucophyte spores;

[0056] 2) Add dilute hydrochloric acid to the nano-sized clay particles, soak for 24 hours, collect the precipitate by centrifugation and then wash it with distilled water 3 times. In a Tween solution with a mass fraction of 1%, a suspension of clay particles is obtained; the humus is dissolved in water, stirred evenly, and kept for 2 hours to obtain a suspension of humus. Settling naturally for 2 hours, taking the supernatant, adding the spores of the pale-colored chitosan spores, stirring evenly, to obtain the spore-water suspension preparation of the pale-colored chitosan spores;

[0057] 3) Adding the water suspension preparation of spores of Chitosan pallenus to the water body containing the mosquitoes, t...

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PUM

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Abstract

The invention provides novel application of bionectria ochroleuca. The bionectria ochroleuca is adopted to prevent and treat a mosquito vector. The invention furthermore provides a method for preventing and treating the mosquito vector by using the bionectria ochroleuca. The method comprises the following steps: 1) culturing bionectria ochroleuca by using a potato glucose agar culture medium at aculture temperature of 20-22 DEG C so as to obtain bionectria ochroleuca spores; 2) collecting the bionectria ochroleuca spores obtained in the step 1), and mixing the bionectria ochroleuca spores with a carrier so as to obtain a bionectria ochroleuca spore preparation; and 3) putting the bionectria ochroleuca spore preparation into a water body with mosquitoes, so as to complete prevention and treatment on the mosquito vector. The novel application has the characteristics of being good in mosquito prevention and treatment effect, simple in prevention and treatment method, and the like.

Description

technical field [0001] The present invention relates to the field of biological control. More specifically, the present invention relates to a new use of Chitosan leucoides. Background technique [0002] Pleurotus leucoides is a kind of heavy parasitic bacteria widely present in soil, which can inhibit tomato gray mold, tomato leaf mold, tomato cotton blight, tomato verticillium wilt, tomato fusarium wilt, poplar rot, poplar Growth of tree wilt and black currant leaf spot, tobacco bacterial wilt and other pathogenic fungi. There are few research reports on the insecticidal activity of this strain. At present, only the toxic activity of the fermentation broth of the strain against Artemia, aphids, diamondback moth, cotton bollworm and beet armyworm has been done. The corrected mortality rate for Artemia is 100%, and the Aphid, diamondback moth, cotton bollworm and beet armyworm had obvious poisoning activity, and the corrected mortality rate was above 78.9%. However, its u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N61/00A01N25/08A01P7/04A01P17/00
CPCA01N61/00A01N25/08A01N63/10
Inventor 刘丽娟公茂庆张本光郭秀霞王海防程鹏刘宏美王怀位张崇星
Owner SHANDONG INST OF PARASITIC DISEASES
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