Drug-modified intraocular lens and preparation method and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
An intraocular lens and drug technology, which is used in drug combinations, pharmaceutical formulations, prostheses, etc.
Inactive Publication Date: 2019-09-10
THE EYE HOSPITAL OF WENZHOU MEDICAL UNIV
View PDF5 Cites 4 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The pathogenesis of PCO is not yet clear, and there is no mature theory to predict its occurrence and development
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0042] The preparation of the intraocular lens of embodiment 1 drug modification
[0043] The medicine used in the present invention is Y27632, empirical molecular formula C 14 h 21 N 3O.2HCl, molecular weight: 320.26. Molecular structure such as figure 1 shown.
[0044] The modification material used in the present invention is polylactic acid-glycolic acid copolymer, linear molecular formula [C 3 h 4 o 2 ] x [C 2 h 2 o 2 ] y , lactic acid: glycolic acid 50:50, molecular weight: 24,000-38,000, molecular structure formula such as figure 2 shown.
[0045] 1. Preparation of main reagents
[0046] (1) Y27632 solution: 1 mg of Y27632 powder was added to 78.04 μl of sterilized double distilled water to prepare a concentration of 40 mM, and stored at 4°C.
[0047] (2) 1% PLGA solution: 1 mg of PLGA powder was dissolved in 0.1 ml of chloroform, and stored at -20°C.
[0048] 2. Intraocular lens simulation material modification
[0049] Take out the intraocular lens, ...
Embodiment 2
[0052] The drug effect of the intraocular lens modified by the drug of embodiment 2
[0053] 1.1 In vitro release test
[0054] Take sterile 1.5ml centrifuge tubes, add 1.0ml of sterilized PBS to each tube, place the drug-modified IOL in PBS, and shake on a constant temperature shaker at 37°C at a frequency of 100rpm for 15min, 30min, 1h, At 2h, 4h, 8h, 12h, 24h, and 48h, take out 200ul of the solution to a sterile centrifuge tube, then add 200ul of sterilized PBS, and put it back on the shaker. After 48 hours of testing, all samples were collected for testing. After zeroing the UV spectrophotometer equipment with PBS, carry out absorbance scanning at 200-800nm wavelength on the solution of Y27632 gradient dilution (40μM, 20μM, 10μM, 5μM, 2.5μM, 1.35μM, 0.625μM, 0.3125μM, 0.15625μM) , used to make the standard curve of drug standards, the measurement sequence is from low concentration to high concentration. Then carry out the absorbance scanning of 200-800nm wavelength ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
thickness
aaaaa
aaaaa
Login to view more
Abstract
The invention discloses a drug-modified intraocular lens and a preparation method and application thereof. According to the intraocular lens, by researching the effect of a signal path on proliferation and migration of lens epithelial cells, a drug with a prevention and treatment effect on posterior capsule opacification after cataract surgery is screened out. It is found that an inhibitor of a ROCK signal path has an influence on the proliferation and migration of the lens epithelial cells, and the drug-modified intraocular lens is provided by improving a drug feeding mode. The intraocular lens can relatively controllably release the explosive drug in the early stage; moreover, by slowly releasing the drug in the later stage, the intraocular lens integrally has the advantages of great biocompatibility and good prevention and treatment effect.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a drug-modified intraocular lens and its preparation method and application. Background technique [0002] Posterior capsular opacity (PCO), also known as posterior capsular opacity, is the main cause of vision loss after cataract surgery, which seriously affects the curative effect of surgery. Studies have shown that the incidence of PCO 3 years after cataract surgery is significantly increased, but there is no significant difference between the incidence of PCO 3 years and 5 years after surgery. Fong et al. conducted a study on 1495 patients and found that the incidence of PCO in 3 years after cataract surgery was about 38.5%, and the analysis of related influencing factors found that young doctors, rounded-corner designed intraocular lens (Intraocular lens, IOL) and Higher incidence of PCO was associated. In addition, another report stated that the proportion of Nd-YAG laser pos...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more