Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Karelinia caspica(Pall.)Less. plasma membrane Na+/H+ reversal transport protein and encoding gent and application thereof

A technology of anti-transporter and floristry, applied in the field of genetic engineering, can solve problems such as unclear function

Active Publication Date: 2019-09-10
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current knowledge about SOS1-mediated Na + The role of efflux in salinity tolerance in Huachai still remains unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Karelinia caspica(Pall.)Less. plasma membrane Na+/H+ reversal transport protein and encoding gent and application thereof
  • Karelinia caspica(Pall.)Less. plasma membrane Na+/H+ reversal transport protein and encoding gent and application thereof
  • Karelinia caspica(Pall.)Less. plasma membrane Na+/H+ reversal transport protein and encoding gent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 Huahuachai Na + / H + Cloning of Antiporter Gene KcSOS1

[0053] The 2-week-old Huachai tissue culture seedlings with consistent growth were moved to the culture box (20cm×15cm×10cm), cultured with Hoagland nutrient solution for 4 weeks, added 150mM NaCl stress treatment for 24h, cut the leaves, rinsed with sterile water, and placed Blot dry water on sterile filter paper, weigh 100mg, grind fully in liquid nitrogen, extract total RNA according to the instructions of TaKaRa MiniBEST Plant RNA Extraction Kit kit, measure the concentration by Quawell5000 nucleic acid protein analyzer, and synthesize the first strand of cDNA according to the TaKaRaPrimeScriptRTase cDNA first strand synthesis kit Instructions for reverse transcription to synthesize cDNA.

[0054] Search the cloned plant plasma membrane Na according to the NCBI database + / H + The nucleotide sequence of antiporter gene SOS1 was found by DNAMAN 8.0 software, and the core degenerate primers K1 a...

Embodiment 2

[0070] Example 2 plasma membrane Na + / H + Prediction and Analysis of Structural Features of Antiporter KcSOS1

[0071] The amino acid sequence of Huahuachai KcSOS1 and the amino acid sequences of other plants in GenBank were analyzed by DNAMAN8.0 software and Blast (https: / / blast.ncbi.nlm.nih.gov), and it was found that KcSOS1 is closely related to the dicotyledonous Artemisia fragrans and Chrysanthemum and Arabidopsis SOS1 protein homology were 88%, 87% and 55% ( Figure 2A ). Detection of KcSOS1 protein using TMHMMServer v.2.0 (http: / / www.cbs.dtu.dk / services / TMHMM / ) and HMMTOP2.0 (http: / / www.enzim.hu / hmmtop / html / submit.html) The transmembrane region and topology are predicted. The results showed that its N-terminus contained 12 transmembrane regions, its C-terminus contained a long hydrophilic tail, and contained a cyclic nucleotide (cNMP) binding domain at 738-828aa ( Figure 2B ). Therefore, Huahuachai KcSOS1 belongs to the plasma membrane Na + / H + Antiporter.

Embodiment 3

[0072] Example 3 plasma membrane Na + / H + Phylogenetic tree analysis of antiporter gene KcSOS1

[0073] In order to analyze the genetic relationship between Huahuachai KcSOS1 gene and other plant SOS1 genes, the phylogenetic tree analysis was carried out using MEGA 6.0 software. The results showed that KcSOS1 was closely related to dicotyledonous plants AjSOS1 and chrysanthemum CcSOS1, but relatively distant to monocotyledonous plants such as wheat TaSOS1 and rice OsSOS1 ( image 3 ). This suggests that KcSOS1 encodes plasma membrane Na + / H + Antiporter.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a karelinia caspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein and an encoding gent and application thereof. An amino acid residue sequence of the kareliniacaspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein KcSOS1 is shown in SEQ ID No.2 in a sequence table; a sequence of the gene encoding the karelinia caspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein is shown in SEQ ID No.1. It is proved through an experiment that when a karelinia caspica(Pall.)Less. KcSOS1 gene is transferred into tobacco, the saltresistanceof the transgenic tobacco can be significantly improved. The provided karelinia caspica(Pall.)Less. KcSOS1 gene can be used for improving the salt stress resistance performance of crops, and has a wide application prospect in the field of salt tolerance inheritance improvement for the crops and fine forage grass.

Description

technical field [0001] The present invention relates to the technical field of genetic engineering, in particular, to a kind of Huahuachai plasma membrane Na + / H + Antiporter KcSOS1 and its encoding gene and application. Background technique [0002] Drought and soil salinization are the main environmental factors restricting plant growth and development. There are about 1 billion hectares of saline-alkali land in the world, of which my country's saline-alkali land accounts for about 10% of the world's total area (Wang Zunqin et al., 1993; Rozema and Flowers, 2008). aggravating, seriously restricting my country's agricultural and animal husbandry production and ecological environment construction (Guo et al., 2013, 2015). Therefore, under the background of the continuous expansion and frequency of saline-alkali land, how to effectively control and reduce soil salinization has become an urgent problem to be solved. [0003] The main strategy of plant cells to resist salt ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8273
Inventor 郭强孟林张晨毛培春田小霞张国芳
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com