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Application of fecal calprotectin and lactoferrin detection to diagnosis of intestinal inflammation diseases of moschus berezovskii

A technology for inflammatory diseases and calprotectin, applied in disease diagnosis, resistance to vector-borne diseases, measurement devices, etc., can solve problems such as antibiotic abuse and affecting normal immune function

Active Publication Date: 2019-09-10
BEIJING FORESTRY UNIVERSITY
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  • Description
  • Claims
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Problems solved by technology

This way of diagnosis and treatment often leads to misdiagnosis and the abuse of antibiotics. Long-term medication will also cause the resistance of the musk deer to antibiotics and other drugs, affecting the normal immune function of the body.

Method used

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  • Application of fecal calprotectin and lactoferrin detection to diagnosis of intestinal inflammation diseases of moschus berezovskii
  • Application of fecal calprotectin and lactoferrin detection to diagnosis of intestinal inflammation diseases of moschus berezovskii
  • Application of fecal calprotectin and lactoferrin detection to diagnosis of intestinal inflammation diseases of moschus berezovskii

Examples

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Embodiment 1

[0023] Example 1. Establishment of a method for evaluating intestinal inflammatory diseases of forest musk deer by detection of fecal calprotectin and lactoferrin

[0024] 1. Materials and methods

[0025] 1. Animals

[0026] From June to June 2016, all forest musk deer of the same age that could be used as experimental subjects in the two musk deer farms of Baoji, Shaanxi, and Dajikang were used as research objects, with a total of 79 musk deer. Among them, there are 29 female individuals and 49 male individuals.

[0027] Fecal samples should be marked and sealed within 1 hour after collection, and stored in a refrigerator at 2-4°C. Grind, weigh, subpackage, and extract fecal calprotectin. After extraction, place it in a freezer at -20°C for fecal calprotectin. ELISA detection.

[0028] 2. Extraction and determination of fecal protein substances

[0029] The extraction method of forest musk deer excrement proteins refers to the method of Peters et al. and DiagnosticLabor...

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Abstract

The invention discloses an application of fecal calprotectin and lactoferrin detection to diagnosis of intestinal inflammation diseases of moschus berezovskii, and provides an application of a substance for detecting the contents of calprotectin and lactoferrin in the feces of the moschus berezovskii to the preparation of products for assessing the intestinal inflammation diseases of the moschus berezovskii. The application verifies that the intestinal diseases of the moschus berezovskii need to select the indexes having high diagnostic value for inflammation diseases, such as calprotectin andlactoferrin. The application provides certain basis for the clinic diagnosis of the intestinal inflammation diseases of captive moschus berezovskii.

Description

technical field [0001] The invention relates to the application of detection of fecal calprotectin and lactoferrin in the diagnosis of forest musk deer intestinal inflammatory diseases in the field of biotechnology. Background technique [0002] Forest musk deer are small and medium-sized herbivores, and all the energy needed for life activities comes from the energy obtained from the digestion and absorption of herbal food. This requires the musk deer to have a strong digestive system, especially the intestines. The enteric inflammatory disease of forest musk deer has always been the technical bottleneck of large-scale artificial breeding, which seriously limits the rapid development of artificial musk deer (Luo Xi, 2014; He Chiming et al., 2011; Xu et al., 2003; He et al., 2014; Wei et al., 2016). Therefore, strengthening the research on the prevention and control of forest musk deer diseases and fundamentally solving the disease problems that plague the artificial large...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/065G01N2333/79Y02A50/30
Inventor 周俊彤徐尚华胡德夫
Owner BEIJING FORESTRY UNIVERSITY
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