SNP molecular marker combination for identifying species of puffers

A technology of molecular markers and species identification, which is applied in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of hybrid toxicity risks, large differences in economic value, human danger, etc. The typing operation is simple and fast, the typing accuracy is high, and the benefit effect is guaranteed

Pending Publication Date: 2019-09-17
CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the breed and source of the hybrid puffer fish are unknown, it is very likely that the hybrid species will have a risk of toxicity, and eating poisonous puffer fish by mistake is very

Method used

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  • SNP molecular marker combination for identifying species of puffers
  • SNP molecular marker combination for identifying species of puffers
  • SNP molecular marker combination for identifying species of puffers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] One of the 90 fugus from different populations was arbitrarily selected as a species identification object for species identification. The specific identification steps include:

[0049] Step 1, obtaining the genome DNA of the puffer from the fin rays, muscles, or blood of a puffer to be evaluated;

[0050] Step 2: Utilize the puffer genome DNA obtained in step 1 to construct a genome-wide SNP microarray chip, and obtain the genotypes of multiple SNP molecular marker sites of puffer puffer.

[0051] Step 3, using bioinformatics software to analyze the genotypes of the multiple SNP molecular marker sites of Puffer puffer to be evaluated according to the Mendelian genetic law, analyze and obtain the typing information of Puffer puffer to be evaluated, and determine the Identification of the species affiliation of puffer puffer.

[0052] If the genotypes of the corresponding SNP loci are completely consistent with the genotype information listed in Fugu001-Fugu022, it can b...

Embodiment 2

[0058] Randomly select one of the 90 puffer fishes from different populations as the seed identification object to carry out puffer puffer seed identification. The specific identification steps include:

[0059] Step 1, obtaining the genome DNA of the puffer fish from the fin rays, muscles, or blood of a fugu fry to be evaluated;

[0060] Step 2: Utilize the puffer genome DNA obtained in step 1 to construct a genome-wide SNP microarray chip, and obtain the genotypes of multiple SNP molecular marker sites of puffer puffer.

[0061] Step 3, using bioinformatics software to analyze the genotypes of multiple SNP molecular marker sites of puffer puffer fry to be evaluated in step 2 according to the Mendelian genetic law, analyze the typing information of puffer puffer fry to be evaluated, and judge The species affiliation of puffer puffer species to be identified.

[0062] If the genotypes of the corresponding SNP loci are completely consistent with the genotype information listed...

Embodiment 3

[0068] Species identification of the parents of hybrid puffer puffer, the specific identification steps include:

[0069] Step 1, obtaining the genome DNA of the puffer from the fin rays, muscles, or blood of a puffer to be evaluated;

[0070] Step 2, constructing a whole-genome SNP microarray chip using the puffer genome DNA obtained in step 1, and obtaining the genotypes of multiple SNP molecular marker sites of the puffer puffer;

[0071] Step 3. Use bioinformatics software to analyze the genotypes of multiple SNP molecular marker sites of Pufferfish to be evaluated according to the Mendelian genetic law, and analyze the typing information of Pufferfish to be evaluated, such as its corresponding SNP The genotype of the site partly matches any of the genotype information listed in Fugu001~Fugu022 or Fugu023~Fugu044 or Fugu045~Fugu066 or Fugu067~Fugu088 or Fugu089~Fugu110, and partly matches Fugu001~Fugu022 or Fugu023~Fugu044 or Fugu045 ~Fugu066 or Fugu067~Fugu088 or Fugu089...

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Abstract

The invention discloses an SNP molecular marker combination for identifying species of puffers. The SNP molecular marker combination comprises an SNP molecular marker combination for identifying takifugu bimaculatus, an SNP molecular marker combination for identifying takifugu flavidus, an SNP molecular marker combination for identifying takifugu niphobles, an SNP molecular marker combination for identifying takifugu rubripes and an SNP molecular marker combination for identifying takifugu obscurus. The invention further discloses a method for identifying the species of the puffers, the species of puffer fries and the species of parents of hybridized puffers. The species of the puffers are identified, so that the situation that detected puffers belong to the species of legitimated edible puffers can be guaranteed, and the benefits of breeding households and food safety are guaranteed; and the species of the puffer hybrids are identified, so that the information of parent species of the bred puffer groups can be clearly traced. The SNP molecular markers are large in number, the genome coverage rate is high, the subtyping operation is simple and quick, and the subtyping accuracy rate is high.

Description

technical field [0001] The invention relates to the field of puffer germplasm identification, in particular to a combination of SNP molecular markers for puffer species identification. Background technique [0002] Pufferfish, belonging to Osteichthyes, Actinopterygii, and Tetraodontiformes, is a kind of fish with delicious meat, high nutritional and economic value, and has both edible and medicinal properties. sex. The body of pufferfish is cylindrical, gray-brown on the back and white on the ventral surface. The markings on the back and sides of the body vary with the species. There are 54 species of pufferfish in my country, of which 21 are edible. Edible puffers are mainly concentrated in the Tetraodontoidei suborder (Tetraodontoidei), and the common puffers include Takifugu bimaculatus (Takifugu bimaculatus), Takifugu rubripes (Takifugu rubripes), and Fugu (Takifugu rubripes) in the family Tetraodontidae. Takifugu obscurus, Takifugu flavidus, Takifugu niphobles, Taki...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 张瀚元许建
Owner CHINESE ACAD OF FISHERY SCI
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