A kind of starch-based amphiphilic self-assembly carrier material and its preparation method and application

Abstract

The invention discloses a starch-based amphiphilic self-assembled carrier material, its preparation method and application. The molecular structure of the starch-based carrier material is shown below, and the molecular weight of the starch-based carrier material is 1.25×10 6 ~1.01×10 8 g / mol, the degree of substitution of carboxymethyl group is 0.20-0.27, the degree of substitution of fatty acid is 0.007-0.21, and the content of M cell targeting peptide CKSTHPLSC is 0.04-11.97%. The starch-based carrier material of the present invention is protonated and insoluble in water under the condition of stomach pH (pH=1.2), deprotonated and slowly soluble in water under the condition of small intestine pH (pH=6.8), and has good gastrointestinal pH responsiveness ; It can spontaneously form stable micelles through hydrophilic-hydrophobic interactions in aqueous solution, and efficiently embed hydrophobic active substances; and it can specifically recognize and bind to M cells to achieve oral M cell-targeted delivery of functional factors.

Description

technical field [0001] The invention relates to a starch-based amphiphilic self-assembled carrier material and its preparation method and application, in particular to a starch-based amphiphilic self-assembled carrier material with the ability to spontaneously form micelles in aqueous solution, pH response and M cell targeting It can realize the loading, controlled release and targeted delivery of M cells to hydrophobic nutritional functional factors. Background technique [0002] With the continuous improvement of social development and people's living standards, as well as the evolution of dietary structure and disease spectrum, people pay more and more attention to nutrition and health. In addition to basic nutritional functions, people's consumption of food pays more attention to the internal quality of dietary nutritional supplements, physiological regulation, and health promotion. Therefore, a large number of functional proteins and peptides, polysaccharides and oligo...

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Problems solved by technology

The amphiphilic carrier material constructs bilayer liposome microcapsules to embed hydrophilic proteins through the method of water-in-oil-in-water, but it needs to add PEG to stabilize the nanoparticles, and the encapsulation efficiency of the carrier material for the target protein is comparable Low, only 30-50%

Moreover, PEG itself has good water solubility and water absorption, which can improve the ability of tablets to release drugs in tablet preparations, and it is difficult to achieve controlled and sustained release of drugs; PEG molecules can change the biomembrane structure of various cells and have certain effects on cells. damage

In Targeted delivery of chitosan nanoparticles to Peyer's patch using M cell-homing peptide selected by phage display technique (Biomaterials, 2010, 31(30): 7738-7747), Yoo et al. used NHS / EDC catalyzed acylation method to shorten CKS9 Peptides are grafted onto chitosan molecular chains to synthesize hydrophilic M cell-targeting carrier materials, but chitosan is easily soluble in acidic environments, which is not conducive to protecting the functional activity of active substances in the gastrointestinal tract

[0005] At present, most of the carrier materials for M cell targeting are hydrophilic or amphiphilic carrier materials that can construct bilayer liposomes, which are suitable for embedding hydrophilic substances, while M cell targeting carriers for hydrophobic active substances less material

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