Insect virus and sex pheromone microcapsule and preparation method thereof
A technology of insect virus and pheromone, applied in the field of insect virus and sex pheromone microcapsule and its preparation
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[0042] A method for preparing insect virus and sex pheromone microcapsules, the preparation method comprising: uniformly mixing microcapsules containing insect virus and insect sex pheromone to obtain a mixture, mixing the mixture with a chitosan solution, and filtering to obtain Insect virus and sex pheromone microcapsules. It can be that the microcapsules containing insect virus and the insect sex pheromone solution are uniformly mixed to obtain a mixed solution, the mixed solution is mixed with a chitosan solution, and filtered to obtain insect virus and sex pheromone microcapsules;
[0043] It can be that the mixture is added into the chitosan solution, stirred, and the chitosan coating of the microcapsules (complete coating by electrostatic bonding), filtration (can be centrifugal filtration three times), to obtain insect virus and sex pheromone microenvironment. capsule.
[0044] In the present invention, the insect virus may be an insect virus capable of infecting inse...
Embodiment 1
[0054] A preparation method of insect virus and sex pheromone microcapsules, comprising the following steps:
[0055] Step 1: Add 0.04g of insect virus dry powder to 30mL of milk fat powder water mixture containing whey protein, stir and mix evenly to form mixture A;
[0056] Step 2: Add mixture A to 63.5mL sodium alginate solution, stir and mix evenly to form mixture B;
[0057] Wherein, in mixture B, the mass fraction of milk fat powder containing whey protein is 4%, and the mass fraction of sodium alginate is 2.5%;
[0058] Step 3: Take mixture B and spray all of mixture B into the calcium chloride solution, and stir while spraying to obtain mixture C; specifically, use the peristaltic pump of the spray dryer to absorb at a speed of 2.5-5mL / min Mixture B, the feed liquid is atomized by a nozzle with an inner diameter of 0.5 mm, and 800-3000 mL of calcium chloride solution with a mass fraction of 2% is placed under the nozzle. The calcium chloride solution is equipped with ...
Embodiment 2
[0064] A preparation method of insect virus and sex pheromone microcapsules, comprising the following steps:
[0065] Step 1: Add 0.04g of insect virus dry powder to 30mL of milk fat powder water mixture containing whey protein, stir and mix evenly to form mixture A;
[0066] Step 2: Add mixture A to 65mL sodium alginate solution, stir and mix evenly to form mixture B;
[0067] Wherein, in mixture B, the mass fraction of milk fat powder containing whey protein is 4%, and the mass fraction of sodium alginate is 1%;
[0068] Step 3: Take mixture B and spray all of mixture B into the calcium chloride solution, and stir while spraying to obtain mixture C; specifically, use the peristaltic pump of the spray dryer to absorb at a speed of 2.5-5mL / min For mixture B, the feed liquid is atomized by a nozzle with an inner diameter of 0.5 mm, and 800-3000 mL of calcium chloride solution with a mass fraction of 2% is placed under the nozzle. The calcium chloride solution is equipped with ...
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