Nucleic acid extraction, amplification and test reagent card

Abstract

The invention relates to the technical field of biological test, in particular to a nucleic acid extraction, amplification and test reagent card, comprising a reagent card body, as well as a liquid packet assembly, a sample holding region, a driving assembly, a nucleic acid extraction region, a nucleic acid amplification region, a nucleic acid test region and a microchannel system which are arranged on the reagent card body; a sample tube is fitted in the sample holding region; the sample holding region is also provided with a piercing assembly used to pierce the sample tube; the microchannel system allows for communication of the driving assembly with the nucleic acid extraction region, the nucleic acid amplification region and the nucleic acid test region; each of microchannels between the driving assembly and the nucleic acid extraction region, nucleic acid amplification region and nucleic acid test region is provided with a valve, such that the driving assembly can be communicated independently with any one of the nucleic acid extraction region, the nucleic acid amplification region and the nucleic acid test region. The technical problem of the prior art is solved that nucleic acid extraction, amplification and test steps are complex to operate independently and are susceptible to pollution.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a nucleic acid extraction, amplification and detection integrated reagent card. Background technique [0002] At present, nucleic acid detection has been widely used in the field of clinical medicine. For example, it is commonly used for virus nucleic acid detection and SNP (single nucleotide polymorphisms) detection. Nucleic acid detection is generally divided into three steps: nucleic acid extraction, nucleic acid amplification, and nucleic acid detection. The current market The nucleic acid detection equipment on the Internet can generally only perform the two steps of nucleic acid amplification and nucleic acid detection, while nucleic acid extraction requires manual operation or the use of additional equipment, which makes the entire nucleic acid detection process a long cycle, cumbersome operation and high cost. Moreover, the detection results are also easily a...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a nucleic acid extraction, amplification and detection integrated reagent card to alleviate the technical problems in the prior art that the three steps of nucleic acid extraction, amplification and detection are cumbersome and complicated to operate independently, and are prone to contamination

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