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Extraction method of inonotus obliquus polysaccharide

A technology of Inonotus obliquus and an extraction method, applied in the field of polysaccharide extraction, can solve the problems of waste of extraction solvent, low extraction rate, long extraction time, etc., and achieve the effects of avoiding the loss of polysaccharide and mild reaction conditions

Pending Publication Date: 2019-09-24
湖北亿彤实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in addition to the traditional water extraction method, the common extraction methods of polysaccharides are also commonly used to extract polysaccharides, such as ultrasonic method, acid extraction method, alkali extraction method and enzyme extraction method; water extraction method requires higher temperature and is easy to damage the The biological activity of Inonotus fusiformis polysaccharides has the disadvantages of long extraction time and low extraction efficiency; acid extraction and alkaline extraction use the principle of concentration gradient to gradually diffuse the active ingredients in the fiber tissue of plant herbal medicines into the extraction solvent , but due to the barrier effect of the plant cell wall, the extraction time is often too long, and a large amount of extraction solvent is wasted, and the extraction rate is low. The method of solvent extraction may also be that the extracted polysaccharide contains impurities such as heavy metals.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for extracting polysaccharides from Inonotus obliquus, specifically comprising the steps of:

[0030] (1) the screened Inonotus obliquus is powdered after freeze-drying to obtain Inonotus obliquus dry powder;

[0031] (2) Put the Inonotus obliquus dry powder into the extraction kettle, then add 90% ethanol in the extraction kettle, reflux and extract 2 times, discard the supernatant, and the remaining Inonotus obliquus raw materials are for subsequent use;

[0032] (3) The raw material of Inonotus obliquus obtained through step (3) is added to purified water, the ratio of solid to liquid is 20mL / g, the pH of the suspension is adjusted to 7.5, and after ultrasonic treatment at normal temperature for 2h, it is separated by suction filtration to obtain The extract and the leached Inonotus obliquus, the obtained extract is concentrated under reduced pressure;

[0033] (4) Add the mixture of cellulase and pectinase in the concentrated extract obtained through step ...

Embodiment 2

[0039] A method for extracting polysaccharides from Inonotus obliquus, specifically comprising the steps of:

[0040] (1) the screened Inonotus obliquus is powdered after freeze-drying to obtain Inonotus obliquus dry powder;

[0041] (2) Put the Inonotus obliquus dry powder into the extraction kettle, then add 90% ethanol in the extraction kettle, reflux and extract 4 times, discard the supernatant, and the remaining Inonotus obliquus raw materials are for subsequent use;

[0042] (3) The raw material of Inonotus obliquus obtained through step (3) is added to purified water, the ratio of solid to liquid is 20mL / g, the pH of the suspension is adjusted to 8.5, and after ultrasonic treatment for 4 hours at normal temperature, it is separated by suction filtration to obtain respectively The extract and the leached Inonotus obliquus, the obtained extract is concentrated under reduced pressure;

[0043](4) Add pectinase and papain mixture in the concentrated extract obtained throug...

Embodiment 3

[0049] A method for extracting polysaccharides from Inonotus obliquus, specifically comprising the steps of:

[0050] (1) the screened Inonotus obliquus is powdered after freeze-drying to obtain Inonotus obliquus dry powder;

[0051] (2) Put the Inonotus obliquus dry powder into the extraction kettle, then add 90% ethanol in the extraction kettle, reflux and extract 3 times, discard the supernatant, and the remaining Inonotus obliquus raw materials are for subsequent use;

[0052] (3) The raw material of Inonotus obliquus obtained through step (3) is added to purified water, the ratio of solid to liquid is 20mL / g, the pH of the suspension is adjusted to 8, and after ultrasonic treatment for 3 hours at normal temperature, it is separated by suction filtration to obtain respectively The extract and the leached Inonotus obliquus, the obtained extract is concentrated under reduced pressure;

[0053] (4) add cellulase, pectinase and papain mixture in the concentrated extract obtai...

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Abstract

The invention relates to an extraction method of inonotus obliquus polysaccharide. A high-voltage pulse electric field-assisted composite enzyme extraction method is mainly adopted for extracting inonotus obliquus powder multiple times, and the influence of the high-temperature environment of a traditional extraction method on the biological activity of the inonotus obliquus polysaccharide is overcome, and the extraction rate of the inonotus obliquus polysaccharide is improved.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a method for extracting polysaccharides from Inonotus obliquus. Background technique [0002] Inonotus obliquus belongs to the phylum Fungi, Basidiomycotina, Phytomycetes, Phytomycetes, Inonotus rotaceae, and Inonotus obliquus. At present, the chemical composition of Inonotus obliquus mainly includes lanolin-type steroids Compounds, polysaccharides, polyphenols, lignin, melanin, alkaloids, tannin compounds, folic acid derivatives, and aromatic vanillic acid and syringic acid, etc. Inonotus obliquus is an important species in Russia, Poland, Japan and other countries common folk medicines. At present, relevant researchers have carried out various studies on various active ingredients in Inonotus obliquus, hoping to gradually apply them in the prevention and treatment of malignant tumors, diabetes, cardiovascular diseases, liver diseases and AIDS. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 黄仁杰约翰.古斯塔夫.卡尔松杰森.陈
Owner 湖北亿彤实业有限公司
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