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A mixed flora of microorganisms that promote plant growth

A technology for promoting plant growth and mixing flora, applied in plant growth regulators, plant growth regulators, microorganisms, etc., can solve problems such as affecting the quality of surface water and groundwater, reducing the quality of agricultural products, and increasing planting costs.

Active Publication Date: 2021-02-26
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to achieve this goal, technicians in the planting industry often blindly use excessive fertilizers, especially chemical fertilizers, which will not only increase planting costs and waste resources, but also cause problems such as soil compaction and acidification of cultivated land and decline in the quality of agricultural products. Non-point source pollution that seriously affects the quality of surface water and groundwater

Method used

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  • A mixed flora of microorganisms that promote plant growth
  • A mixed flora of microorganisms that promote plant growth
  • A mixed flora of microorganisms that promote plant growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Microbial mixed flora promotes growth of hydroponic plants

[0018] The specific implementation steps are as follows:

[0019] Flavobacterium pectinovorum CGMCC12362 (denoted as bacteria A), Lysobacter ruishenii CGMCC10136 (denoted as bacteria B), Enterobacter mori CGMCC10322 (denoted as bacteria C), were inoculated separately Into LB medium, cultured with shaking at 30°C until the bacterial concentration is O.D. 600 ≈2.0, mixed according to the volume ratio of 1:1:1, 1:2:1, 1:1:2, 2:1:1 respectively, and at the same time, two kinds of bacteria were selected and mixed in the volume ratio of 1:1, and then Take the single-bacteria culture solution of three kinds of bacteria. Each of the above-mentioned culture solutions was prepared according to the 10 6 The final microbial concentration of CFU / ml was added to the rhizosphere of rapeseed in the hydroponic system, and the culture was continued for 1 week. In the present invention, a blank control group is also set, and...

Embodiment 2

[0025] Microbial mixed flora promotes soil culture plant growth

[0026] The specific implementation steps are as follows:

[0027] Flavobacterium pectinovorum CGMCC12362 (denoted as bacteria A), Lysobacter ruishenii CGMCC10136 (denoted as bacteria B), Enterobacter mori CGMCC10322 (denoted as bacteria C), were inoculated separately Into LB medium, cultured with shaking at 30°C until the bacterial concentration is O.D. 600 ≈2.0, mixed according to the volume ratio of 1:1:1, 1:2:1, 1:1:2, 2:1:1 respectively, and at the same time, two kinds of bacteria were selected and mixed in the volume ratio of 1:1, and then Take the single-bacteria culture solution of three kinds of bacteria. Each of the above-mentioned culture solutions was prepared according to the 10 6 The final microbial concentration of CFU / ml was added to the rhizosphere of rapeseed in the soil culture system, and the culture was continued for 1 week. Under the same conditions, an equal amount of water was added to...

Embodiment 3

[0033] Determination of Auxin Secreting Ability of Microbial Strains

[0034] The specific implementation steps are as follows:

[0035] Flavobacterium pectinovorum CGMCC12362 (denoted as bacteria A), Lysobacter ruishenii CGMCC10136 (denoted as bacteria B), Enterobacter mori CGMCC10322 (denoted as bacteria C), were inoculated separately Into LB medium, cultured with shaking at 30°C until the bacterial concentration is O.D. 600 ≈2.0. Take 100 mL of the three bacterial cultures respectively, and use high-performance liquid chromatography-tandem mass spectrometry to measure the auxin (IAA) content therein, as shown in Table 3 below.

[0036] Table 3 Results of strain secretion auxin experiment

[0037] Growth hormone secretion capacity (mg / L) Bacteria A 306.5 Bacteria B 356.1 Bacteria C 328.2

[0038] It can be known from Example 3 that all three strains have the ability to secrete auxin, which can promote plant growth and increase crop yield ...

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Abstract

The invention discloses a microbial mixed flora for promoting plant growth, which belongs to the technical field of agricultural microorganisms. The mixed microbial flora is composed of Flavobacterium pectinosa, Lysobacterium jyosensii, and Enterobacter mulberry; the mixed microbial flora is prepared by the following method: combining Flavobacterium pectinophilus, Lysobacillus joruishenii, Enterobacter mulberry were inoculated into LB medium respectively, and cultured with shaking at 30°C until the bacterial concentration was O.D. 600 ≈2.0, and then mixed according to the volume ratio of 1:1:1. The mixed flora has a synergistic effect on plant growth, which is better than the growth-promoting effect of a single strain or a pairwise combination of strains, and has stronger adaptability in the actual soil environment.

Description

technical field [0001] The invention relates to a mixed flora composed of multiple microbial strains, in particular to a method for the flora to promote plant growth, and belongs to the technical field of agricultural microorganisms. Background technique [0002] Promoting yield increase is the primary task of modern high-efficiency agriculture. In order to achieve this goal, technicians in the planting industry often blindly use excessive fertilizers, especially chemical fertilizers, which will not only increase planting costs and waste resources, but also cause problems such as soil compaction and acidification of cultivated land and decline in the quality of agricultural products. Non-point source pollution that seriously affects the quality of surface water and groundwater. Therefore, controlling the amount of chemical fertilizers is a practical need to promote cost savings, energy conservation and emission reduction, and is of great significance to ensure national food...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/20C12R1/01
CPCA01N63/00C12N1/20
Inventor 李强许菡亓文钰韩琪
Owner LINYI UNIVERSITY
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