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Biomarkers related to cervical squamous cell carcinoma and application thereof

A biomarker, cervical squamous cell carcinoma technology, applied in cervical squamous cell carcinoma-related biomarkers and their application fields, can solve the problems of low diagnostic value of lymph node metastasis, and achieve the effect of early diagnosis

Active Publication Date: 2019-10-15
泗水县人民医院
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, the most meaningful marker for clinical treatment is squamous cell carcinoma antigen, but its specificity and sensitivity are only 60%, and its diagnostic value for judging whether there is lymph node metastasis is low

Method used

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  • Biomarkers related to cervical squamous cell carcinoma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] Example QPCR detection of the expression of WDR5B and TRIOBP in cervical squamous cell carcinoma

[0044] 1. Sample collection

[0045] 35 cases of cervical squamous cell carcinoma tissues and corresponding paracancerous tissues were collected. All cases did not receive any immunosuppressant treatment, radiotherapy and chemotherapy before operation.

[0046] 2. RNA sample preparation and quality analysis

[0047] Extraction of tissue total RNA using TRIZOL method

[0048] 1) Mince the tissue with scissors, add 1ml Trizol, shake on the shaker for 1min; place at room temperature for 10min to completely decompose the ribosomes.

[0049] 2) Add 200 μl of chloroform (chloroform), close the cap tightly, shake vigorously for 15 seconds, and let stand at room temperature for 10 minutes.

[0050] 3) Centrifuge at 11000 rpm for 15 minutes at 4°C.

[0051] 4) Transfer the water sample layer to a new centrifuge tube, add 500 μl of isopropanol; after inverting to mix well, let s...

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PUM

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Abstract

The invention discloses biomarkers related to cervical squamous cell carcinoma and application thereof. The biomarkers are WDR5B and TRIOBP. According to the biomarkers related to the cervical squamous cell carcinoma and application thereof, differential expression of the WDR5B and the TRIOBP in the cervical squamous cell carcinoma is discovered for the first time, in view of this, the invention discloses application of the WDR5B and the TRIOBP in preparing products for diagnosing the cervical squamous cell carcinoma and products for diagnosing the cervical squamous cell carcinoma comprising reagents for detecting expression levels of the WDR5B and TRIOBP.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to biomarkers related to cervical squamous cell carcinoma and applications thereof. Background technique [0002] The incidence and mortality of cervical cancer ranks fourth among malignant tumors in the world (Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J] . CA Cancer J Clin, 2018, 68(6):394-424.). More than 500,000 women are diagnosed with cervical cancer every year, and the disease causes more than 300,000 deaths worldwide (Cohen PA, Jhingran A, Oaknin A, et al. Cervical cancer[J]. Lancet, 2019, 393(10167): 169 -182.), and about 85% of the patients are from developing countries or underdeveloped regions (Small W, Bacon MA, Bajaj A, et al. Cervical cancer: A global health crisis[J]. Cancer, 2017, 123 (13):2404-2412.). The main cause of cervical cancer is persistent infect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574C12N15/11
CPCC12Q1/6886G01N33/57411
Inventor 李文梅张永生
Owner 泗水县人民医院
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