Unlock instant, AI-driven research and patent intelligence for your innovation.

Molecular marker snp732 of Hu sheep mc4r gene and its application

A technology of molecular markers and sheep body, which is applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc. It can solve problems such as protein retention, protein folding or intracellular transport errors, and MC4R protein reduction.

Active Publication Date: 2022-04-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the phenotypic effects caused by mutations in the MC4R gene, mutations in the gene can be divided into five types: ① causing defects in MC4R protein synthesis, or accelerating its degradation, manifested as a significant decrease in the amount of MC4R protein; ② causing protein folding or intracellular transport 3. Although the protein can be correctly integrated in the cell membrane, the ability to bind to the ligand is reduced; 4. Causes signal transduction disorders; 5. The effect caused by the mutation is not yet clear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker snp732 of Hu sheep mc4r gene and its application
  • Molecular marker snp732 of Hu sheep mc4r gene and its application
  • Molecular marker snp732 of Hu sheep mc4r gene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0085] Embodiment 3 test result

[0086] PCR Amplification of MC4R Gene Fragment in Hu Sheep

[0087] The results of PCR amplification of Hu sheep MC4R gene showed that when primers 1F and 1R, 2F and 2R were used for amplification, specific amplified bands could appear between 750-1000bp, which corresponded to the expected molecular weight (respectively corresponding to primers 1F and 1R The 862bp of product, the 670bp of primer 2F and 2R product) are identical ( figure 1 ).

[0088] Mutation Analysis of PCR Amplified Product of MC4R Gene in Hu Sheep

[0089] Table 5 shows the mutation analysis results of PCR amplification products of Hu sheep MC4R gene.

[0090] Table 5 SNPs and mutation types and methods of MC4R gene in Hu sheep

[0091]

[0092]

[0093] Use primer pair 1F and 1R (the amplified product is 862bp, see the SEQ ID NO:6 in the sequence listing), 2F and 2R (the amplified product is 670bp, see the SEQ ID NO:7 in the sequence listing) in Hu mutton A tota...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the field of molecular markers, in particular to the molecular marker SNP732 of Hu sheep MC4R gene and its application. The application in marker-assisted breeding further relates to a method for screening the body size traits of Hu sheep and its application in molecular marker-assisted breeding of Hu sheep.

Description

technical field [0001] The invention relates to the field of molecular markers, in particular to a SNP molecular marker of the MC4R gene of Hu sheep, a pair of related primers, a kit, and their application in molecular marker-assisted breeding of Hu sheep, and further relates to a method for screening the body size of Hu sheep Methods of traits and their application in molecular marker-assisted breeding of Hu sheep. Background technique [0002] Melanocortin receptor-4 (MC4R) is a class of peptides secreted by the ventromedial nucleus of the hypothalamus, involved in the regulation of body weight and energy balance, and expressed by multiple neuron populations in the central nervous system. Unlike other members of the MCR family, MC4R does not exist in the adrenal cortex, hair follicles, and placenta, but exists in large quantities in various regions of the central nervous system, including the cerebral cortex, thalamus, hypothalamus, brainstem, and spinal cord. The MC4R of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 姜俊芳蒋永清宋雪梅吴建良
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES