Preparation method of multi-phase solid culture medium

Abstract

The invention discloses a preparation method of a multi-phase solid culture medium. The preparation method includes following steps: step 1, preparing various phase solutions and coagulators differentin property, putting a culture dish body with an opening upward, and enabling the culture dish body to be in a state with the opening open; step 2, pouring a first phase solution into a culture dishfrom the opening, and allowing coagulation of the first phase solution after the first phase solution fills the lower side of the culture dish; step 3, pouring a second phase solution into the culturedish from the opening, and allowing coagulation of the second phase solution after the second phase solution fills above a first phase; repeating the step 2 and the step 3 to obtain the multi-phase solid culture medium. The first phase solution fully fills the lower portion of the culture dish after cooling and coagulating, a two-phase culture medium can be obtained by directly pouring the secondphase solution onto the first phase solution for coagulation, and a partition is not needed, so that operation is simpler and more convenient; a step of pulling the partition away is omitted, so thatdamage to the culture medium is avoided. The invention relates to the field of plant cultivation.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for preparing a multiphase solid medium. Background technique [0002] Under the normal growth state of plants, during the continuous growth of the plant root system, the root system continues to extend downward from the relatively loose soil layer on the surface to the relatively dense soil in the deep layer. In order to simulate the growth status of this kind of plant, two-phase or even multi-phase Phase medium, two-phase or multiphase medium means that the hardness or nutrients of each phase are different. Taking the two-phase medium as an example, the preparation process is usually as follows: put the culture dish flat, first put a partition in the culture dish, pour the first phase solution and the second phase solution on both sides of the partition at the same time, and cool and solidify , and then remove the separator to obtain a solid medium with two phases. T...

AI Technical Summary

Problems solved by technology

The above method has the following disadvantages. First, the clapboard is plugged into the petri dish and requires high fit with the petri dish. Otherwise, the first phase solution and the second phase solution are exchanged at the junction, which will affect the first phase after cooling. The properties of the solid medium and the second phase medium; second, when the separator is taken away, the first phase solid medium and the second phase solid medium will be pulled, and it is easy to culture the first phase solid medium and the second phase solid medium damage to the base and affect the results of simulation experiments

Third, the opening depth of the petri dish is small, and it is easy to overflow when pouring the solution

Images