Method for extracting flavonoid and chlorogenic acid from chrysanthemum by ionic liquid aqueous two-phase

A technology of ionic liquids and flavonoids, which is applied in the direction of pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of adverse environmental effects, cumbersome operations, and high costs, and achieve environmental friendliness, high extraction efficiency, and easy operation. easy effect

Inactive Publication Date: 2019-11-01
上海工微所科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent extraction method uses traditional organic solvents, which are highly volatile and have adverse effects on the environment; the macroporous resin adsorption method is cumbersome and time-consuming; the cost of instruments required for supercritical fluid extraction is relatively high

Method used

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  • Method for extracting flavonoid and chlorogenic acid from chrysanthemum by ionic liquid aqueous two-phase
  • Method for extracting flavonoid and chlorogenic acid from chrysanthemum by ionic liquid aqueous two-phase
  • Method for extracting flavonoid and chlorogenic acid from chrysanthemum by ionic liquid aqueous two-phase

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preparation example Construction

[0043] Preparation of standard curve

[0044] Configuration of standard stock solution of 5 standard substances (luteolin, apigenin-7-O-glucoside, chlorogenic acid, isochlorogenic acid A and isochlorogenic acid C): Weigh 1mL standard substance and dissolve in In a 10mL volumetric flask, dilute to volume after ultrasonic dissolution with methanol, and store at low temperature in the dark. Pipette 100 microliters of each standard stock solution into a 10mL volumetric flask, dilute with methanol to the mark, and set aside.

Embodiment 1

[0046] A method for extracting flavonoids and chlorogenic acids in chrysanthemum with ionic liquid two-phase aqueous phase, comprising the steps of:

[0047] (1) Sample pretreatment: take an appropriate amount of chrysanthemum, grind it to powder, and place it in an oven at 45°C for 6 hours.

[0048] (2) Add 1 L of [Bmim]Br ionic liquid solution with a concentration of 0.5 mol / L to 20 g of chrysanthemum samples, shake it well to make it evenly mixed, add 350 g of ammonium sulfate to the system, shake it enough to dissolve all the inorganic salts in the system , forming a homogeneous suspension; the ultrasonic power is 100W, ultrasonic at room temperature for 5 minutes, taken out, and after standing for 30 seconds, a two-phase aqueous system is formed. The upper layer is an ionic liquid phase rich in flavonoids and chlorogenic acids, and the lower layer An aqueous phase in which inorganic salts are dissolved. Transfer the system to a separatory funnel, discharge the lower phas...

Embodiment 2

[0057] A method for extracting flavonoids and chlorogenic acids in chrysanthemum with ionic liquid two-phase aqueous phase, comprising the steps of:

[0058] (1) Sample pretreatment: take an appropriate amount of chrysanthemum, grind it to powder, and dry it in an oven at 35°C for 7 hours.

[0059] (2) Add 1 L of [Bmim]Cl ionic liquid solution with a concentration of 0.75 mol / L to 50 g of chrysanthemum samples, shake it well to make it evenly mixed, add 400 g of sodium dihydrogen phosphate to the system, shake it enough to dissolve all the inorganic salts in the In the system, a uniform suspension is formed. Take it out after ultrasonic power of 200W, ultrasonic at room temperature for 10min, and let it stand for 30s. The solution will automatically separate into layers to form a two-phase aqueous system. A similar ionic liquid phase, and the lower layer is an aqueous phase in which inorganic salts are dissolved. Transfer the system to a separatory funnel, discharge the lower...

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Abstract

The invention relates to a method for extracting flavonoid and chlorogenic acid from chrysanthemum by ionic liquid aqueous two-phase. The method for extracting the flavonoid and the chlorogenic acid from the chrysanthemum by the ionic liquid aqueous two-phase comprises the following steps that (1) an ionic liquid solution is added to chrysanthemum powder, after the mixture is mixed uniformly, inorganic salt is added to a system, the mixture is fully mixed to form a uniform suspension; (2) the suspension is ultrasonically extracted, and then the mixture is placed silently to form a two-phase system, the upper layer is an ionic liquid phase rich in the flavonoid and the chlorogenic acid, and the lower layer is an aqueous phase with the dissolved inorganic salt, after the liquid separation, an upper layer solution is obtained, that is, a concentrated solution rich in the flavonoid and the chlorogenic acid; and (3) ionic liquid in the concentrated solution rich in the flavonoid and the chlorogenic acid is separated, and extract rich in the flavonoid and the chlorogenic acid is obtained. The method for extracting the flavonoid and the chlorogenic acid from the chrysanthemum by the ionicliquid aqueous two-phase has the characteristics of simple operation, high phase separation speed, high extraction efficiency, low cost and environmental friendliness.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting flavonoids and chlorogenic acids from chrysanthemum with ionic liquid two-phase Background technique [0002] Chrysanthemum is one of the commonly used medicinal and edible plants. It has the functions of dispersing wind, protecting liver, improving eyesight, clearing fire and detoxifying. There are many kinds of active ingredients in chrysanthemum, such as chlorogenic acids, terpenes, flavonoids, polysaccharides and other small molecule active ingredients. Among them, chlorogenic acids and flavonoids account for a large proportion, and the flavonoids and chlorogenic acids mainly include chlorogenic acid, luteolin, isochlorogenic acid B, isochlorogenic acid A, apigenin-7- O-glucuronide and other ingredients. Among them, the flavonoid luteolin has anti-inflammatory, antioxidant activity, anti-tumor and neuroprotective effects. Chloroge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/287
CPCA61K36/287A61K2236/333A61K2236/51A61K2236/55
Inventor 李爽宁准梅姜宇齐江长源张光华
Owner 上海工微所科技有限公司
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