Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for excavating testicular differential proteins of Tibetan sheep before and after sexual maturity based on DIA technology

A digging, Tibetan sheep technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as poor reproducibility of peptide identification

Active Publication Date: 2019-11-01
GANSU AGRI UNIV
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the conventional data-dependent acquisition mode, the randomness of precursor ion acquisition will cause poor reproducibility of peptide identification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for mining differential proteins in Tibetan sheep testes before and after sexual maturity based on DIA technology, comprising the following steps:

[0031] Step A. Tissue collection

[0032] In the described step A, the testicular tissues described in this patent come from male Tibetans with similar genetic background (same paternal mother) at two key developmental stages before sexual maturity (3 months old) and after sexual maturity (1 year old). Sheep: After slaughtering the sheep, take out the testis tissue, wash the external blood stains with PBS and peel off the buffy coat, cut it into small tissues of 0.5cm×0.5cm×0.5cm, and quickly freeze them in liquid nitrogen for protein samples preparation of

[0033] Step B. Protein sample preparation

[0034] Add lysate (7M urea, 2% SDS, 0.1% PMSF, 65mMDTT) to the testicular tissue, and ultrasonically lyse; after centrifuging at 4°C and 14000rpm for 30min, draw the supernatant, and use the BCA protein quantificat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for excavating testicular differential proteins of Tibetan sheep before and after the sexual maturity based on a DIA (Data Independence Acquisition) technology. The method comprises the following steps: tissue collection, preparation of a protein sample, creation of a spectral database, qualitative and quantitative analysis of proteins and screening of differentialexpressed proteins. According to the method disclosed by the invention, testicular tissues of 3-month-old and 1-year-old Tibetan sheep are selected; the spectral database is analyzed and created by using a conventional DDA mass-spectrometric detection technology; and then, spectral data acquisition is performed by the DIA method, and the proteins in the sample are qualitatively and quantitativelyanalyzed, thus obtaining the differential expressed proteins in the testicles of the Tibetan sheep. The method can be used to realize quantitative screening of the differential expressed proteins inthe testicles of the Tibetan sheep, which provides a very important theoretical basis to subsequent researches on a molecular mechanism of testicular development or spermatogenesis of the Tibetan sheep under a high-altitude condition from proteomics.

Description

technical field [0001] The invention relates to the technical field of DIA quantitative methods, in particular to a method for mining differential proteins in the testes of Tibetan sheep before and after sexual maturity based on the DIA technology. Background technique [0002] As one of the three primitive sheep breeds in China, Tibetan sheep are mainly distributed in Tibet, Qinghai, Gansu, Sichuan and other places on the Qinghai-Tibet Plateau above 3000m above sea level. Sustainable development and maintaining ecological balance play a very important role. However, due to long-term grazing and lack of supplementary feeding, their production performance is low, their fecundity is low, and their sexual maturity is late, which seriously limits the reproduction of Tibetan sheep flocks, and thus restricts the economic and social development of pastoral areas. Proteins are the main participants and executors in the life activities of organisms. Therefore, it is of great signif...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 马友记李讨讨王霞张宏豫
Owner GANSU AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com