An Image Enhancement Method in a Digital Slice Scanner

Abstract

The invention discloses an image enhancement method in a digital slice scanner, comprising the following steps: step 1, obtaining a thumbnail navigation map of a physical slice, and identifying the sample area and the position of the cover glass; step 2, according to the sample area and the CCD The corresponding relationship between the field of view and the thumbnail navigation map obtains the scanning field of view of the sample; step 3, finds the non-sample area in the cover glass area; step 4, obtains the image of the enhanced image; Image, image enhancement processing is performed by enhancing the image of the image. The technical scheme of the present invention effectively improves the uniformity of image brightness, removes dark spots in the image caused by dust, obtains high-quality images, and ensures the uniformity of brightness of obtained high-resolution full-information digital slices.

Description

Technical field[0001]The present invention relates to an image enhancement method in a digital slice scanner, which belongs to the field of image processing.Background technique[0002]The digital slice scanner is a device that combines computer technology with micro-optical techniques to digitize physical slice. The process of slice is the use of industrial CCD cameras to collect microscopic enlarged images, registering images, forming digital slice images of high resolution full information.[0003]In a digital slice scanner, the micro-optical system is the basis of image clarity, image contrast, and image brightness. A important part of the micro-optical system is an optical portion. The optical basic principle is that the light emitted by the light source passes through the concentrating light into a parallel beam, and then the beam is transmitted to the physical slice, passes through the objective mirror or the like to the camera CCD. The light is generated by the concentrating mir...

AI Technical Summary

Problems solved by technology

[0004] However, in existing digital slice scanners, the light beam transmits through the slice and is refracted by the slide glass, which further changes the parallelism of the light beam and aggravates the unevenness of the image brightness; the light beam enters the In the CCD of industrial cameras, tiny dust sometimes exists in these parts, and the tiny dust will form obvious dark spots on the image, thus affecting the quality of the picture

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