A method for detecting amantadine, rimantadine and memantine residues in animal-derived food

Abstract

The present disclosure provides a method for detecting the content of amantadine, rimantadine and memantine in animal-derived food, the steps comprising: (1) taking a sample and adding a reconstituted solution of trichloroacetic acid and acetonitrile, vortexing, and centrifuging after ultrasonic treatment , remove the supernatant A and save it, add the reconstituted solution of trichloroacetic acid and acetonitrile to the remaining residue, vortex, centrifuge after sonication, take out the supernatant B and combine it with the supernatant A, and then blow dry to obtain the sample Extract residue; (2) re-solution vortex shaking, add C18, PAS and neutral alumina, sonicate and centrifuge, filter the supernatant through a filter membrane to obtain the sample to be tested; (3) The contents of amantadine, rimantadine and memantine in the test samples were detected by ultra-high performance liquid chromatography tandem mass spectrometer. The method effectively solves the problems of high interference and low recovery rate during simultaneous detection of amantadine, rimantadine and memantine, saves cost and improves work efficiency.

Description

technical field [0001] The present disclosure relates to the field of food safety, in particular to a method for detecting amantadine, rimantadine and memantine residues in animal-derived food. Background technique [0002] With the rapid progress of society, people's living standards have been greatly improved, and animal food has gradually become a rigid need in daily life. People's market demand for animal food has led to the expansion of food animal breeding. The blind expansion of the low-scale breeding volume has led to the complexity of food animal diseases and the confusion of veterinary drug market supervision. Memantine and others are used for food animals, but these drugs are not approved for veterinary use. [0003] In 2005, the U.S. Food and Drug Administration (FDA) has ordered the prohibition of the abuse of human antiviral drugs in livestock and poultry. At the same time, the Ministry of Agriculture Announcement No. 560 has also banned the use of antiviral ...

AI Technical Summary

Problems solved by technology

[0004] However, the existing detection methods for antiviral drug residues in livestock and poultry meat are relatively old, and the detection items and matrices are relatively single. Each antiviral drug detection method is different, and the instruments and reagents used have different degrees of In addition, there are large differences in the detection range and precision of each method. Even some antiviral drugs still lack detection methods, which has caused problems such as high detection cost, long cycle, and great difficulty.

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