Flat plate flow cavity capable of simultaneously applying static pulling force and flow shearing force

Abstract

The invention discloses a flat plate flow cavity capable of simultaneously applying static pulling force and flow shearing force. The flow cavity comprises a base (1) and a cover plate (2) which are mutually connected, wherein a flow cavity groove (3) is arranged in the base (1); a placing groove (4) is arranged at the bottom of the flow cavity groove (3); two ends of the placing groove (4) are respectively provided with a fixing groove (401) and a sliding groove (402); a flexible film (5) is arranged in the placing groove (4); one end of the flexible film (5) is provided with a first clampingpin (501) buckled into the fixing groove (401); the other end of the flexible film (5) is provided with a second clamping pin (502) buckled into the sliding groove (402); and a plurality of the sliding blocks (6) are arranged in the sliding groove (402) at the outer side of the second clamping pin (502). The flow cavity can simultaneously load static traction force and flow shearing force on cells and adjust the loaded stress.

Description

technical field [0001] The invention relates to a flat-plate flow cavity, in particular to a flat-plate flow cavity which applies static tension and flow shear force simultaneously. Background technique [0002] Due to the complexity of the physiological structure of biological tissues and organs and the diversity of physiological environments in vivo, somatic cells are often in a complex mechanical environment and can be simultaneously subjected to various forms of mechanical stimulation including stretch stress and shear stress. ; and the biomechanical environment is of great significance for cells to carry out normal life activities and changes under pathological conditions. Therefore, it has become an important and routine research in the field of modern cell biomechanics to apply mechanical devices to cells cultured in vitro to simulate the stress stimulation of the in vivo environment. means. [0003] At present, the main device for studying the influence of stress on...

AI Technical Summary

Problems solved by technology

As a result, the existing devices cannot truly simulate the corresponding growth environment and cannot meet the research needs

[0004] In addition, due to the high level of sterility required in the simulated environment for cell research, the conventional flat flow structures used to apply different stress stimuli cannot be combined with each other to achieve the effect of simultaneously applying multiple stress stimuli under the condition of ensuring the aseptic effect. , which increases the difficulty of applying multiple stimulus stresses in the flat flow chamber

Moreover, the combination of the stress stimulation structure in the flat flow chamber will also cause a large expansion of the cavity structure, which will seriously increase the flow cost of the medium in the cavity and reduce its flow stability, making it difficult to apply pressure on the cells. The various stimulus stresses form obstacles to each other, which affects the authenticity of the simulation

Therefore, existing flat flow chambers cannot simultaneously apply multiple stimulatory stresses to cells

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