Sheep seminal fluid diluent, and preservation method

A preservation method and diluent technology, which are applied in the field of diluent and preservation of sheep semen, can solve the problems of restricting the promotion of diluent of sheep semen, high requirements for refrigerated storage conditions, and few types of diluent, so as to protect the survival of sperm and reduce the Cell freezing point, the effect of improving sperm motility

Active Publication Date: 2019-11-19
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few types of diluents for sheep semen, and the requirements for cold storage c

Method used

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  • Sheep seminal fluid diluent, and preservation method
  • Sheep seminal fluid diluent, and preservation method
  • Sheep seminal fluid diluent, and preservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A diluent of sheep semen, each 100mL of the diluent comprises the following components: Y-27632 2HCl, Vitamin E 4mL, fresh egg yolk 20mL, glycerol 4mL, fructose 1.26g, citric acid monohydrate 1.72g, tris(hydroxymethyl)aminomethane 3.53g, vitamin C 0.5g, bovine serum albumin 0.5g, penicillin sodium 15000IU, streptomycin sulfate 15000IU, the balance is double distilled water. Prepare 5 groups of dilutions, group 1: blank control, Y-276322HCl 0mg; group 2: Y-276322HCl 0.1601mg / 100mL (corresponding to the molar concentration of 5mM); group 3: Y-276322HCl 0.6405mg / 100mL (corresponding to the molar concentration of 20 mM); Group 4: Y-276322HCl 1.6013 mg / 100 mL (corresponding to a molar concentration of 50 mM); Group 5: Y-276322 HCl 3.2026 mg / 100 mL (corresponding to a molar concentration of 100 mM).

Embodiment 2

[0031] The preservation method of embodiment 2 sheep semen

[0032] 1. Collection of semen

[0033] The semen collection site is fixed in the breeding sheep farm attached to the experimental station. Before semen collection, the rams are put into the shed, and the sturdy frame used to restrain the ewes is placed in the shed. Before semen collection, the shed is cleaned. Find a healthy, disease-free, strong and docile ewe in the ewe house, and fix it on the restraint frame to ensure that the female animal is in the male animal's field of vision, so as to arouse the male sheep's sexual desire. Clean the fake vagina, sperm collection cup and glass rod, install the prepared sperm collection cup on one end of the fake vagina, then pour 50-60 ℃ hot water into the interlayer of the fake vagina, and install the valve with piston; Take a small amount of vaseline with a glass rod, and evenly apply a layer on the inner tube. After blowing and pressurizing, one end of the vaseline coated...

Embodiment 3

[0068] A dilution of sheep semen, the dilution includes the following components per 100 mL: Y-27632 2HCl 0.6405 mg, vitamin E 3.5 mL, fresh egg yolk 19 mL, glycerin 3.5 mL, fructose 1.25 g, citric acid monohydrate 1.7 g, Tris 3.5g, vitamin C 0.4g, bovine serum albumin 0.4g, penicillin sodium 10000IU, streptomycin sulfate 10000IU, the balance is double distilled water.

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Abstract

The invention relates to a sheep seminal fluid diluent, and a preservation method. Each 100ml of the sheep seminal fluid diluent comprises Y-27632 2HCl 0.16 to 3.20mg, vitamin E 3.5 to 4.5ml, fresh yolk 19 to 21ml, fructose 1.2 to 1.3g, citric acid monohydrate 1.7 to 1.8g, trimethylolaminomethane 3.5 to 3.6g, vitamin C 0.4 to 0.6 g, bovine serum albumin 0.4 to 0.6 g, penicillin sodium 10000 to 15000 IU, streptomycin sulfate 1000 to 15000 IU, glycerin 3.5 to 4.5ml, and the balance double distilled water. The sheep seminal fluid diluent is capable of prolonging preservation time of sheep seminalfluid under low temperature conditions, and increasing the quality of preserved sperm.

Description

technical field [0001] The invention relates to a diluent of semen, in particular to a diluent of sheep semen and a preservation method. Background technique [0002] my country has a long history of sheep farming, and the germplasm resources of sheep and goats are rich, of which the number of breeding accounts for the largest in the world. Sheep farming is of great significance to the country's modernization development. Sheep farming has huge development prospects in the vast grasslands and rural areas. The rapid development of sheep farming can not only contribute to the country's reform and opening up, but also improve people's living standards. With the continuous advancement of science and technology, the development of sheep farming in my country has also made great progress, but there are also many problems. Compared with developed countries, there is still a big gap, especially in the aspects of large-scale breeding and intensive breeding. In recent years, in order...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 曹鸿国李彩霞唐云书孙寒雪高晋凤涂丹凤卢振涛张苍龙孟祝张子军任春环陈家宏刘亚周建军陈宏权
Owner ANHUI AGRICULTURAL UNIVERSITY
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