A microbial toxicity and biodegradability evaluation method of landfill leachate concentrate and its application

Abstract

The invention discloses a method for evaluating microbial toxicity and biochemical properties of landfill leachate concentrate and its application. The method comprises the following steps: (1) Pseudomonas in the logarithmic growth phase are respectively inoculated into the medium containing the landfill leachate concentrate with concentration gradient as the test group, and the medium inoculated with Pseudomonas is used as the test group. Blank control group, cultivated until TOC and TN in the test group tended to be stable; (2) Microbial toxicity evaluation and / or biodegradability evaluation of landfill leachate concentrate: Determination of Pseudomonas in the test group and the blank control group Growth density, in order to evaluate the microbial toxicity of the landfill leachate concentrate; measure the TOC concentration and TN concentration of the test group and the blank control group before and after cultivation, and calculate their T t and D t , to evaluate the biodegradability of landfill leachate concentrate. The method of the invention is expected to become an important means for the technical research on improving the biochemical property of the landfill leachate concentrate.

Description

technical field [0001] The invention belongs to the technical field of garbage treatment, and in particular relates to a method for evaluating microbial toxicity and biodegradability of a garbage leachate concentrate and an application thereof. Background technique [0002] Landfill leachate membrane concentrate has complex components, high salt content, high concentration of pollutants, poor biodegradability, and serious harm to the environment. At present, for the treatment of landfill leachate membrane dope, the main methods that have been reported are: recharge method, evaporation method, advanced oxidation method, etc. The recharge method will continue to enrich pollutants, especially inorganic salts and refractory organic matter, with the increase of recharge times, while the evaporation method and advanced oxidation method are expensive to achieve harmless treatment of the concentrated liquid, and cannot adapt to the concentration of the membrane. The trend of increa...

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Problems solved by technology

The recharge method will continue to enrich pollutants, especially inorganic salts and refractory organic matter, with the increase of recharge times, while the evaporation method and advanced oxidation method are expensive to achieve harmless treatment of the concentrated liquid, and cannot adapt to the concentration of the membrane. The trend of increasing liquid production

In recent years, the research on advanced oxidation pretreatment combined with biochemical methods to treat landfill leachate membrane dope has been reported, but the key components that affect the biodegradability of membrane dope have not been ascertained and how to match, optimize and reduce the cost of advanced oxidation technology , leading to a very limited role of biochemical methods in the treatment process in practical applications, and there are still technical difficulties in large-scale engineering applications of advanced oxidation composite biochemical combined processes

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