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Preparation method of native copper/rhizoma drynariae-loaded compound brushite

A compound calcium phosphate rock and drynariae technology, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of short half-life, easy to dissociate, high price, etc., to maintain stable release, simple and easy-to-obtain raw materials, Inexpensive effect

Active Publication Date: 2019-12-03
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of these active biomolecules are very expensive, and at the same time have a short half-life and are easy to dissociate, which requires high preparation technology for composite materials, and the material has a short shelf life, which increases the production cost of materials and increases the economic burden of patients in clinical use.

Method used

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  • Preparation method of native copper/rhizoma drynariae-loaded compound brushite
  • Preparation method of native copper/rhizoma drynariae-loaded compound brushite
  • Preparation method of native copper/rhizoma drynariae-loaded compound brushite

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Effect test

Embodiment 1

[0026] A preparation method of natural copper / drynaria rhizome compound brushite, comprising the steps of:

[0027] Step 1. Weigh and sieve 200-mesh β-calcium phosphate powder and calcium phosphate monohydrate powder according to the molar ratio of 1.1:1, put them into a mortar, and grind for 15 minutes to obtain mixed powder A1;

[0028] Step two, prepare 1.5wt% sodium alginate solution, 2wt% CaCl 2 solution and 0.5M citric acid solution containing 1 wt% chitosan, select the drynaria extract and grind it into powder, add it to the sodium alginate solution, stir it evenly, extract it with a syringe and add CaCl dropwise 2 In the solution, microspheres were formed and cross-linked for 4 hours. The microspheres were washed with deionized water, soaked in citric acid solution, washed with deionized water again, and pre-frozen at -80°C for 24 hours. Freeze-dried for 6 hours to obtain calcium alginate microspheres loaded with 3wt% Rhizoma Drynariae extract, powder A1 was added cal...

Embodiment 2

[0032] A preparation method of natural copper / drynaria rhizome compound brushite, comprising the steps of:

[0033] Step 1. Weigh and sieve 200-mesh β-calcium phosphate powder and calcium phosphate monohydrate powder according to the molar ratio of 1.1:1, put them into a mortar, and grind for 15 minutes to obtain mixed powder A1;

[0034] Step 2, prepare 2wt% sodium alginate solution, 4wt% CaCl 2 solution and 0.5M citric acid solution containing 1 wt% chitosan, select the drynaria extract and grind it into powder, add it to the sodium alginate solution, stir it evenly, extract it with a syringe and add CaCl dropwise 2 In the solution, microspheres were formed and cross-linked for 4 hours. The microspheres were washed with deionized water, soaked in citric acid solution, washed with deionized water again, and pre-frozen at -80°C for 24 hours. Freeze-dried for 6 hours to obtain calcium alginate microspheres loaded with 3wt% Rhizoma Drynariae extract, add calcium alginate micros...

Embodiment 3

[0038] A preparation method of natural copper / drynaria rhizome compound brushite, comprising the steps of:

[0039] Step 1. Weigh and sieve 200-mesh β-calcium phosphate powder and calcium phosphate monohydrate powder according to the molar ratio of 1.1:1, put them into a mortar, and grind for 15 minutes to obtain mixed powder A1;

[0040] Step 2: Prepare 2.5wt% sodium alginate solution, 6 wt% CaCl 2solution and 0.5M citric acid solution containing 1 wt% chitosan, select the drynaria extract and grind it into powder, add it to the sodium alginate solution, stir it evenly, extract it with a syringe and add CaCl dropwise 2 In the solution, microspheres were formed and cross-linked for 4 hours. The microspheres were washed with deionized water, soaked in citric acid solution, washed with deionized water again, and pre-frozen at -80°C for 24 hours. Freeze-dried for 6 hours to obtain calcium alginate microspheres loaded with 3wt% Rhizoma Drynariae extract, add calcium alginate micr...

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Abstract

The invention relates to a preparation method of native copper / rhizoma drynariae-loaded compound brushite. The method specifically comprises the following steps: weighing beta-calcium phosphate powderand calcium phosphate monohydrate powder according to a molar ratio of 1.1: 1, grinding the materials to obtain mixed powder A1, adding calcium alginate microspheres loaded with 3wt% of a rhizoma drynariae extract, grinding the materials to obtain mixed powder A2, weighing vinegar-calcined native copper and tri-distilled water according to the ratio of 1: 2, decocting with slow fire until the ratio is 11: 1, and filtering to obtain a filtrate B; and adding the filtrate B into the mixed powder A2 according to a ratio of 2.5: 1, blending to obtain a uniform paste, filling the paste into a mold,curing, and taking out to obtain the composite material. Compared with drugs loaded with growth factors and the like, the price of the material of the invention is lower, the used raw materials are simple and easy to obtain, the drug effect is stable, the product is suitable for large-scale production, and a material system of the composite microsphere is more stable by utilizing the characteristics that the synthesis environment of the brushite is acidic and the calcium alginate microsphere does not expand under the acidic condition.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method of natural copper / drynaria drynaria compound brushite. Background technique [0002] Natural copper has a long history as a medicine for bone healing in our country. According to the statistics of the "Dictionary of Traditional Chinese Medicine Prescriptions", among the 117 prescriptions titled with the word "Jiangu" in the past dynasties, 43 of them used natural copper, accounting for 36.8%. Studies have shown that natural copper is a ferrous mineral pyrite, rich in iron, which is beneficial to the formation of collagen in the early callus of fracture healing, and can also promote the calcification of callus in the middle and late stages. At the same time, natural copper also contains a variety of trace elements necessary for the human body such as copper, zinc, and manganese, which can enhance the activity of various enzymes, promote the for...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/20A61L27/02A61L27/12A61L27/50A61L27/54A61L27/56
CPCA61L27/025A61L27/12A61L27/20A61L27/3637A61L27/50A61L27/54A61L27/56A61L2300/102A61L2300/30A61L2300/412A61L2430/02C08L5/04
Inventor 赵三团裴正军李光大张开丽商文超李朋斌翟江波景爱华李景华
Owner HENAN UNIV OF SCI & TECH
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