Immunochromatography detection card for rapidly detecting phosphorylated Tau protein and preparation method thereof

An immunochromatography and phosphorylation technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of cytoskeleton deformation, loss of function, aggregation, etc., and achieve the effect of high sensitivity, overcoming poor stability, and difficult to agglomerate

Pending Publication Date: 2019-12-03
TIANJIN SAVANT BIOTECH CO LTD
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hyperphosphorylation can lead to deformation and aggregation of various types of cytoskeleton in nerve tissue, and then loss of normal function

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunochromatography detection card for rapidly detecting phosphorylated Tau protein and preparation method thereof
  • Immunochromatography detection card for rapidly detecting phosphorylated Tau protein and preparation method thereof
  • Immunochromatography detection card for rapidly detecting phosphorylated Tau protein and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Preparation of phosphorylated Tau protein immunochromatographic detection card

[0044] 1) Preparation of surface-activated fluorescent latex microspheres:

[0045] N,N'-dilauroyl ethylenediamine sodium diacrylate, polyethylene glycol monolaurate, dodecylbenzenesulfonate and lauroyl glutamate, the weight ratio of the four is 5:6 :1:8.

[0046]①Take the surfactant (including 5 mg of N, N'-dilauroyl ethylenediamine sodium diacrylate, 6 mg of polyethylene glycol monolaurate, 1 mg of dodecylbenzenesulfonate and 8 mg of lauryl Acylglutamate) was added to 0.2mol / L, boric acid-borax buffer solution (containing 0.5wt%-3wt% PEG2000) at pH=8.4, and then 1mL dimethylformamide, 1mg N,N'- Dicyclohexylcarbodiimide and 0.5mg N-hydroxysuccinimide were reacted at a stirring speed of 120r / min;

[0047] ②Take 1mL of fluorescent latex microsphere dispersion containing 1wt% carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and use 10mL, 0.2mol / ...

Embodiment 2

[0078] 1. Preparation of phosphorylated Tau protein immunochromatographic detection card

[0079] 1) Preparation of surface-activated fluorescent latex microspheres:

[0080] N,N'-dilauroyl ethylenediamine sodium diacrylate, polyethylene glycol monolaurate, dodecylbenzenesulfonate and lauroyl glutamate, the weight ratio of the four is 1:4 :2:11.

[0081] ① Take the surfactant (containing 1 mg of N, N'-dilauroyl ethylenediamine sodium diacrylate, 4 mg of polyethylene glycol monolaurate, 2 mg of dodecylbenzenesulfonate and 11 mg of lauryl Acylglutamate) was added to 0.2mol / L, boric acid-borax buffer solution (containing 0.5wt%-3wt% PEG2000) at pH=8.4, and then 1mL dimethylformamide, 1mg N,N'- Dicyclohexylcarbodiimide and 0.5mg N-hydroxysuccinimide were reacted at a stirring speed of 120r / min;

[0082]②Take 1mL of the dispersion of fluorescent latex microspheres containing 1wt% carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and use 1...

Embodiment 3

[0107] 1. Preparation of phosphorylated Tau protein immunochromatographic detection card

[0108] 1) Preparation of surface-activated fluorescent latex microspheres:

[0109] N,N'-dilauroyl ethylenediamine sodium diacrylate, polyethylene glycol monolaurate, dodecylbenzenesulfonate and lauroyl glutamate, the weight ratio of the four is 1:4 :3:6.

[0110] ① Take the surfactant (containing 1 mg of N, N'-dilauroyl ethylenediamine sodium diacrylate, 4 mg of polyethylene glycol monolaurate, 3 mg of dodecylbenzenesulfonate and 6 mg of lauryl Acylglutamate) was added to 0.2mol / L, boric acid-borax buffer solution (containing 0.5wt%-3wt% PEG2000) at pH=8.4, and then 1mL dimethylformamide, 1mg N,N'- Dicyclohexylcarbodiimide and 0.5mg N-hydroxysuccinimide were reacted at a stirring speed of 120r / min;

[0111] ② Take 1 mL of the dispersion containing 1 wt% fluorescent latex microspheres with carboxyl groups on the surface (purchased from Shanghai Zhenzhun Biotechnology Co., Ltd.), and u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to an immunochromatography detection card for rapidly detecting phosphorylated Tau protein. The immunochromatography detection card comprises a test strip, the test stripcomprises a detection line and a quality control line, the detection line is coated with a mouse anti-human phosphorylated Tau protein monoclonal antibody, and the quality control line is coated witha goat anti-rabbit polyclonal antibody. The immunochromatography detection card for rapidly detecting the phosphorylated Tau protein provided by the invention can be used for detecting serum, plasmaand whole blood to diagnose Alzheimer's disease.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and relates to an immunochromatographic detection card for rapidly detecting phosphorylated Tau protein and a preparation method thereof. Background technique [0002] Tau protein is a microscopically related protein, mainly distributed in neurons, followed by glial cells. Under normal circumstances, post-transcriptional phosphorylation of tau is beneficial to the stability of microtubules. However, hyperphosphorylation can lead to deformation and aggregation of various types of cytoskeleton in nerve tissue, and then loss of normal function. [0003] Tau protein has more than 45 phosphorylation sites, most of which are located in the proline-rich region (that is, the phosphorylation site-rich region, mostly in the 172-251 site region) and the C-terminal tail region (mostly in the 368- 441 site region). Hyperphosphorylation in these regions of tau affects its ability to bind microtubules. Mor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533G01N33/543
CPCG01N33/558G01N33/533G01N33/54313
Inventor 林斯柴诗缘胡琛光
Owner TIANJIN SAVANT BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap