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Lactobacillus jensenii and application thereof for preparing vaginal bacteriostatic drugs

A technology of Lactobacillus jannis and drugs, applied in the field of microorganisms, can solve the problems of not having the ability to inhibit vaginal pathogenic bacteria, and achieve the effect of superior vaginal epithelial cell adhesion, active and stable biological characteristics, and inhibition of Candida albicans

Active Publication Date: 2019-12-06
广东强基药业有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to provide a specific Lactobacillus jansii to solve the technical problem in the prior art that conventional Lactobacillus jansii does not have the ability to inhibit vaginal pathogenic bacteria

Method used

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  • Lactobacillus jensenii and application thereof for preparing vaginal bacteriostatic drugs
  • Lactobacillus jensenii and application thereof for preparing vaginal bacteriostatic drugs
  • Lactobacillus jensenii and application thereof for preparing vaginal bacteriostatic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 (isolation and inoculation, purification, enrichment culture of Lactobacillus jansnii 'RD-0135 flora)

[0054] 1. Isolation and inoculation of Lactobacillus jannis RD-0135 flora: Use two sterile cotton swabs to collect the secretions on the side wall of the subject's vagina, and inoculate them with different concentrations within 24 hours in the prepared MRS Petri dish of culture medium, and label the information, place the Petri dish in an anaerobic tank, and put CO 2 Put the gas-producing bag in a 37°C incubator and incubate for more than 48 hours.

[0055] 2. Purification and enrichment culture of Lactobacillus jansnii strain RD-0135: Count the colonies according to their different shapes (surface, edge, etc.) and size. Those with the same shape and size are counted as one, and the inoculation loop is used to pick a single colony A few bacteria were inoculated into the MRS solid medium according to the "slash method" to obtain isolated and purified sing...

Embodiment 2

[0056] Embodiment 2 (identification and preservation of Lactobacillus jansnii RD-0135 bacterial strain)

[0057] 1. Culture characteristics, staining microscopic examination and morphological characteristics: the colonies obtained after culture are as attached figure 1 , the colonies are generally round, with neat edges, raised in the middle, milky white and opaque; take a smear of the pure culture of the bacteria for Gram staining, and the results are shown in the attached figure 2 , showing Gram-positive, short rod-shaped, blunt-rounded ends, arranged in short chains. The results showed that the isolated strains were preliminarily determined to be Lactobacillus.

[0058] 2. Identification of 16SrDNA gene sequence: DNA was extracted with a bacterial genomic DNA extraction kit, and primers 27F (5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') were used for PCR amplification. The PCR product was subjected to gel electrophoresis to determine the 16S rDNA ge...

Embodiment 3

[0072] Embodiment 3 (determination of metabolites of Lactobacillus jansnii RD-0135)

[0073] 1. Determination of lactic acid content in metabolites of Lactobacillus jansnii RD-0135: use high performance liquid phase method (0.005M sulfuric acid aqueous solution (0.28ml sulfuric acid (98%)-1000ml water, pH is about 2.1) to cultivate this bacterial strain for 24-48h to ferment The lactic acid of liquid is detected, and the result indicates that lactic acid (L / D lactic acid sum) is about 30mg / mL, which is far higher than the lactic acid content (about 15mg / mL) of Lactobacillus delbrueckii (commercially available). The lactic acid detection spectrum of the present invention can be found in attached Figure 4 .

[0074] 2. Determination of hydrogen peroxide content in the metabolites of Lactobacillus jansii RD-0135: semi-quantitative determination of hydrogen peroxide was carried out according to the peroxidase method of Mcgroarty et al., and the isolated and identified Lactobacil...

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Abstract

The invention provides lactobacillus jensenii and application thereof for preparing vaginal bacteriostatic drugs. The lactobacillus jensenii is deposited in the China General Microorganisms CollectionCenter of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 14112. Research on the metabolic performance of the lactobacillus jensenii provided by theinvention discovers that, compared to ordinary lactobacillus jensenii, the strain has high lactic acid production capacity, high hydrogen peroxide production capacity, high bacteriostatic ability topathogenic bacteria, and outstanding vaginal epithelial cell adhesion ability. Based on the new properties discovered above, the invention determines a new use of the lactobacillus jensenii for preparing the vaginal bacteriostatic drugs, and the vaginal bacteriostatic drugs can realize treatment of a variety of bacterial vaginal diseases. The bactericidal agent provided by the invention has obvious effects on bacterial vaginal diseases, is safe and non-toxic, has good stability, and can be stored for a long time. The invention also relates to the use of the lactobacillus jensenii in drugs forprevention and / or treatment of gynecological diseases.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and further relates to the regulation of vaginal micro-ecological environment and the treatment of bacterial vaginal diseases, in particular to a kind of Lactobacillus jannis and its application for preparing vaginal bacteriostatic drugs. Background technique [0002] There are a variety of microorganisms in the vagina of healthy women, and they form a vaginal micro-ecological system that restricts, coordinates and dynamically balances with the host and the environment. The vaginal flora of healthy women is mainly composed of Lactobacillus, including Lactobacillus plantarum, Lactobacillus jansii, Lactobacillus gasseri, Lactobacillus crispatus, Lactobacillus vaginal, Lactobacillus jansii, etc. Under normal circumstances, Lactobacillus can protect the vagina. When the Lactobacillus in the vaginal microecology is disturbed, it can lead to the invasion of pathogenic bacteria and cause vaginiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31A61K35/747A61P15/02A61P31/04A61P31/10C12R1/225
CPCA61P15/02A61P31/04A61P31/10A61K2035/115C12R2001/225C12N1/205Y02A50/30
Inventor 许少燕赵湘江陈贵浩李煜龙许锐鹏张佳冬黄旭藩潘立
Owner 广东强基药业有限公司
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