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A kind of Lactobacillus crispatus and its application in women's health products

A Lactobacillus crispatus, product technology, applied in bacteria, cosmetics, topical antibacterial agents, etc., can solve the problems of bacterial growth, itching, inflammation and even gynecological inflammation, suffering from gynecological diseases, etc., to achieve high viable bacteria rate, superior vaginal Epithelial cell adhesion and inhibition of Candida albicans

Active Publication Date: 2018-07-06
SUZHOU OSEL BIO PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the cosmetics used in the genitals, the above problems also exist. If they are not properly cared for, they will easily become a breeding ground for bacteria, leading to itching, inflammation and even gynecological inflammation. Therefore, experts recommend not to abuse disinfectants and cosmetics frequently and for a long time. Bacterial imbalance, suffering from gynecological diseases, etc.

Method used

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  • A kind of Lactobacillus crispatus and its application in women's health products
  • A kind of Lactobacillus crispatus and its application in women's health products
  • A kind of Lactobacillus crispatus and its application in women's health products

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Effect test

Embodiment 1

[0057] Embodiment 1, isolation and inoculation, purification, enrichment culture of Lactobacillus crispatus Lc262-1 flora

[0058] 1. Isolation and inoculation of Lactobacillus crispatus Lc262-1 flora: samples were collected using Port. A-Cd system. Use two sterile cotton swabs to collect 1 / 3 of the secretions on the side wall of the subject's vagina, inoculate them in different concentrations on a petri dish containing the prepared Rogosa SL medium within 24 hours, and mark the information. The dish was placed in an anaerobic tank and placed in CO 2 Put the gas-producing bag in a 37°C incubator and incubate for more than 48 hours.

[0059] 2. Purification and enrichment culture of Lactobacillus crispatus strain Lc262-1: Count the colonies according to their different shapes (surface, edge, etc.) and size, and record those with the same shape and size as one, and pick a few of a single colony with the inoculation loop Bacteria are inoculated into MRS solid medium according ...

Embodiment 2

[0060] Embodiment 2, the strain identification of Lactobacillus crispatus Lc262-1 bacterial strain

[0061] 1. Culture characteristics, staining microscopic examination and morphological characteristics: The colonies obtained after cultivation are shown in Figure 1. The colonies are gray-white round, full in the middle, diffuse around, and irregular; take a smear of the pure culture of the bacteria for Gram staining , the result is as figure 2 , showing Gram-positive, short rods, can be connected to long chains; the results of electron microscope analysis are shown in image 3 , under the electron microscope, the strain has no spores, no flagella, no capsule, and the strain size is 26.824×6.667um. The results showed that the isolated strains were preliminarily determined to belong to the genus Lactobacillus.

[0062] 2. Identification of 16SrDNA gene sequence: DNA extraction was carried out with a bacterial genomic DNA extraction kit, and PCR amplification was performed on ...

Embodiment 3

[0064] Embodiment 3, determination of metabolites of Lactobacillus crispatus Lc262-1 strain

[0065] 1. Determination of lactic acid content in metabolites of strains: D-lactic acid detection kit measured the output of D-lactic acid of the strain, and the result was 6.213g / L; the content of L-lactic acid measured by the sensor analyzer was 3.789g / L L, the results are shown in the T0 group data in Table 1 below.

[0066] Table 1 Determination results of lactic acid

[0067] sample

D-lactic acid (g / L)

L-lactic acid (g / L)

T0

6.213

3789

T30

6.334

3.330

T50

6.291

3.225

[0068] 2. Determination of hydrogen peroxide content in bacterial metabolites: semi-quantitative determination of hydrogen peroxide was carried out according to the peroxidase method of Mcgroarty, and the separated and identified Lactobacillus crispatus Lc262-1 was inoculated in H 2 o 2 To identify the MRS-TMB plate, after anaerobic culture at 37°C for 24...

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Abstract

The invention discloses a Lactobacillus crispatus Lc262‑1, a bacterial agent containing the strain and its application in women's health care products. The Lactobacillus crispatus Lc262‑1 is a new strain of the genus Lactobacillus crispatus, The preservation number in the General Microbiology Center of the China Microbiological Culture Collection Management Committee is CGMCC No.6469. The Lactobacillus crispatus Lc262-1 is the dominant bacterium in the vagina, which can stably secrete lactic acid, hydrogen peroxide and other substances to maintain the acidic environment of the vagina. Inhibit the growth of harmful bacteria, safe and non-toxic, stable subculture and expanded culture, and long-term storage, the Lactobacillus crispatus Lc262-1 and bacteria containing the strain are applied to women's health care products such as medical equipment, disinfection products or cosmetics .

Description

technical field [0001] The present invention relates to a new strain of Lactobacillus genus and its application, in particular to a kind of Lactobacillus crispatus, which can regulate vaginal microenvironment and inhibit Gardnerella vaginalis (BV pathogenic bacteria), and at the same time, it has obvious anti-white Candida, a pathogenic bacteria, has great advantages and potential for application in daily women's health care. Background technique [0002] As a product of modern science and technology, medical devices have been widely used in the prevention, diagnosis, treatment, health care and rehabilitation of diseases, and have become an important means of diagnosis and treatment in the field of modern medicine. However, like pharmaceuticals, the use of medical devices carries certain risks. Factors related to risk include: (1) Design factors. Restricted by factors such as scientific and technological conditions, cognitive level, and craftsmanship, there are problems su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61L31/16A61L15/44A61L15/36A61K35/747A61K8/99A61P31/02A61Q19/00A61Q19/10C12R1/225
CPCA61K8/99A61K35/747A61K2800/10A61L15/36A61L15/46A61L31/005A61L31/16A61L2300/404A61Q19/00A61Q19/10C12N1/205C12R2001/225Y02A50/30
Inventor 李建龙
Owner SUZHOU OSEL BIO PHARM
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