Linear scanning-based optical imaging device and imaging method of stripe sequence slice

Abstract

The invention belongs to the technical field of optical imaging, and particularly relates to a linear scanning-based optical imaging device and imaging method of stripe sequence slice. The linear scanning-based optical imaging device and imaging method aims to solve the problem that continuous imaging of the stripe sequence slice cannot be achieved by the prior art. The device comprises a stripe,a stripe coiling device, a stripe supply device, a word line light source, a linear array image sensor and an image processing unit, wherein the stripe is used for placing biological tissues sequenceslices in sequence, the stripe coiling device and the stripe supply device are respectively fixed with two ends of the stripe and are respectively used for rotating and coiling the stripe at a uniformspeed and supplying the stripe, the word line light source and the linear array image sensor are arranged at two sides or the same side of the stripe and are used for scanning the sequence slices under a stripe transmission state, and the image processing unit is used for receiving a series of linear array images sent from the linear array image scanning device and generating a stripe sequence slice image. By the linear scanning-based optical imaging device, the stripe is driven to transmit the slice by the stripe coiling device and the stripe supply device, the strips are sequentially scanned line by line by the linear array image sensor, the sequence image is finally generated by the image processing unit, and the image is high in resolution and low in distortion, and the image production efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of optical imaging, and in particular relates to a strip sequence slice optical imaging device and method based on line scanning. Background technique [0002] Strip serial sectioning refers to cutting biological tissue into continuous serial slices, and using automatic tape collection equipment to collect the slices one by one on the strips. The earliest equipment that uses automatic strips to collect continuous ultra-thin serial slices was designed and implemented by Jeff Litchman's laboratory at Harvard University in the United States. It is mainly used for micro-nano-scale scanning imaging of continuous ultra-thin slices of brain tissue in an electron microscope. , to obtain the three-dimensional structural information of the neural structure. However, only the microstructure information of the tissue is obtained, without the relevant category and function information corresponding to the structure, it ...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned problems in the prior art, that is, the prior art cannot realize continuous imaging of strip sequence slices, the present invention provides a strip sequence slice optical imaging device based on line scanning, the imaging device includes strips, Strip winding device, strip supply device, line light source, line array image sensor, image processing unit;

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