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Antioxidant activity detection and compound application of plant-derived antioxidants

An antioxidant and plant-derived technology, which is applied in the measurement of color/spectral characteristics, can solve the problems of reducing the contraction amplitude and increasing the tension of the duodenal intestine in rabbits, and achieves improved activity, simple and easy detection methods, and maintenance The effect of sustainable development

Active Publication Date: 2022-04-29
HAINAN UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

Arecoline can stimulate M receptors to compensate for the lack of acetylcholine, promote body excitement, and improve learning and memory capabilities; arecoline can significantly increase the tension of the duodenal intestine in rabbits, reduce the contraction amplitude, and do not affect the contraction frequency; in addition, Arecoline has inhibitory effects on Gram-negative and Gram-positive bacteria such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, and Salmonella gallinarum, but there are few reports on antioxidant research

Method used

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  • Antioxidant activity detection and compound application of plant-derived antioxidants
  • Antioxidant activity detection and compound application of plant-derived antioxidants
  • Antioxidant activity detection and compound application of plant-derived antioxidants

Examples

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Embodiment 1

[0024] Example 1: Compound identification of plant-derived antioxidants

[0025] A, antioxidant preparation:

[0026] Accurately weigh 3.1038g of arecoline, add 5mL of distilled water to dissolve, stir gently to make it completely dissolved, add distilled water to make up to 10mL. The completely dissolved and no bubbles were selected as the mother liquor for the identification of antioxidants.

[0027] Accurately weigh 2.5428g norarecoline, add 5mL of distilled water to dissolve, stir gently to make it completely dissolved, then add distilled water to make up to 10mL. The completely dissolved and no bubbles were selected as the mother liquor for the identification of antioxidants.

[0028] B. Identification of antioxidants:

[0029] (1) Preparation of different concentrations of antioxidants: prepare 6 different concentrations of arecoline solutions, namely 0mM arecoline, 0.5mM arecoline (molar mass concentration, that is, 77.595mg arecoline per liter of water), 1mM arecoli...

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Abstract

The invention belongs to the technical field of natural antioxidants and improves a compound type plant source antioxidant and a detection method for antioxidant activity. The present invention found for the first time that the content ratio of each component in the total alkaloids of arecoline is the key factor affecting the antioxidant capacity of arecoline extracts, so creatively carried out compound research on different arecolines, the results showed that arecoline and norarecoline The molar ratio (12.8‑13.3): 1 compounding has obvious synergistic antioxidative effect, and finally the present invention obtains a plant source antioxidant with significantly improved activity. In addition, the detection method of the present invention is simple and easy, and can identify the antioxidant capacity of various plant-derived antioxidants with different compound ratios in a short period of time, which is of great significance for maintaining the sustainable development of antioxidants.

Description

technical field [0001] The invention belongs to the technical field of natural antioxidants, and in particular relates to a compound type plant-derived antioxidant and a method for detecting antioxidant activity. Background technique [0002] Antioxidants are a class of substances that can prevent auto-oxidation or increase the oxidation stability of other substances to a large extent. According to the source, antioxidants can be divided into natural antioxidants and synthetic antioxidants. The former exists in animals, plants or microorganisms, while the latter can only be synthesized by chemical methods. According to the mechanism, antioxidants can be divided into primary antioxidants and secondary antioxidants. The former can capture active free radicals, so they are also called free radical scavenger; they provide hydrogen atoms to free radicals and form relatively stable free radicals themselves; Or directly combined with active free radicals to form a stable free radi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 韦运谢施海涛
Owner HAINAN UNIVERSITY
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