Bacillus licheniformis (BL)3 Silence information regulator (Sir)2-like protein capable of relieving nonalcoholic fatty liver disease (NAFLD) tissue lesions and application of BL3 Sir2 protein

Abstract

The invention relates a bacillus licheniformis (BL)3 Silence information regulator (Sir)2-like protein capable of relieving nonalcoholic fatty liver disease (NAFLD) tissue lesions and application of the BL3 Sir2-like protein, discloses the BL3 Sir2-like protein capable of significantly improving the NAFLD tissue lesions and inhibiting lipid droplet formation and the application of the BL3 Sir2-like protein, and belongs to the technical field of biology. The BL3 Sir2-like protein capable of significantly improving the NAFLD tissue lesions and inhibiting lipid droplet formation is separated andprepared from a BL3 strain, and the strain is obtained by screening through a laboratory. The BL3 Sir2-like protein capable of significantly improving the NAFLD tissue lesions and inhibiting lipid droplet formation is prepared in the mode that a gene is cloned into escherichia coli for expression and purification, the experimental result shows that the protein level related to the processes of lipid metabolism, oxidative stress, insulin resistance and the like are regulated and controlled by regulating genes related to lipid metabolism of hepatocytes and affecting PPAR-gamma and PGC-1alpha passages, thus lipid accumulation in liver tissue is reduced, the metabolic abnormality of NAFLD rats is relieved, a new therapeutic strategy is provided for a probiotic Sir2-like protein used for prevention and treatment of NAFLD, and the BL3 Sir2-like protein is used for application to NAFLD prevention and treatment drugs or foods.

Description

technical field [0001] The invention belongs to the field of new mechanism of probiotic Sir2 protein for treating diseases, more specifically, the invention relates to a Bacillus licheniformis BL3 Sir2-like protein which can significantly improve non-alcoholic fatty liver disease tissue lesions and inhibit the formation of lipid droplets and its application. Background technique [0002] Sir2 (Silence information regulator) protein is mainly located in the nucleolus of cells and is a highly conserved NAD + Dependent protein deacetylase, found in bacteria and mammals. Sir2 protein and NAD + Interaction, on the one hand, the regulation of Sir2 protein function is regulated by NAD + Effects of synthetic and salvage pathways, especially deacetylation of Sir2 protein requires NAD + as a cofactor; on the other hand, NAD + Intermediate products such as nicotine and niacin in the salvage pathway and NADH and some NAD + Metabolites can also regulate the activity of Sir2 protein...

AI Technical Summary

Problems solved by technology

However, as far as the current research is concerned, there are few reports on the biological functions of probiotic Sir2 homologous proteins, and they are still in the initial stage, especially the regulatory functions in metabolism.

However, NAFLD, which is caused by metabolic disorders and other causes found in research, has no specific drugs and treatment strategies so far.

The probiotic Bacillus licheniformis also contained in this study sir2 The homologous gene of Sir2 has a Sir2-like protein. Although it has been found that SIRT1, SIRT3 and SIRT6 of the Sirtuin family in mammals can improve NAFLD, the regulatory effect of prokaryotic Sir2 protein on NAFLD has not been reported, and there is no report on Bacillus licheniformis BL3Sir2 Research on the effect of protein on lipid metabolism in cells, therefore, these are worthy of further research

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