Immunodetection kit and measurement method and preparation method thereof
An immunodetection and kit technology, applied in the field of immunoassay kits, can solve the problems of insufficient release and inaccurate results, and achieve the effects of simple storage, simple preparation method and convenient preparation.
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[0101] (Preparation method of immunoassay kit)
[0102] The method for preparing the above-mentioned immunoassay kit provided by the present invention, such as Figure 5 shown, including the following steps:
[0103] S100, preparing a conjugate carrier;
[0104] S200, preparing immunochromatographic test paper;
[0105] S300, packaged immunoassay kit.
[0106] Among them, such as Figure 6 As shown, in step S100, preparing the conjugate carrier includes:
[0107] S110, combining the ligand and the label to obtain a conjugate;
[0108] S120. Prepare a diluent, disperse the obtained conjugate in the diluent, and dilute the conjugate to a certain concentration;
[0109] S130, fixing the above diluted solution on the solid phase carrier by spraying or smearing, and drying to obtain the conjugate carrier;
[0110] S140, cutting the conjugate carrier into a fixed size, and dispensing it in a sealed chamber.
[0111] Wherein, in step S200, preparing immunochromatography test ...
Embodiment 1A
[0122] Embodiment 1AFP fluorescent microsphere immunoassay kit
[0123] 1. Structure of AFP fluorescent microsphere immunoassay kit:
[0124] It includes an immunochromatographic test strip and a conjugate solid phase carrier, the immunochromatographic test strip includes a bottom plate, and the bottom plate is sequentially connected with a first sample pad, a second sample pad, a nitrocellulose membrane and an absorbent pad, The T-line position of the nitrocellulose membrane is coated with AFP antibody, and the C-line position is coated with goat anti-mouse IgG; the label in the conjugate solid-phase carrier is europium ion fluorescent microspheres, the ligand is AFP antibody, and the solid-phase carrier is Glass fiber, the conjugate solid phase carrier is placed in a centrifuge tube.
[0125] 2. Preparation of AFP fluorescent microsphere immunoassay kit:
[0126] Reagents and consumables:
[0127] Table 1
[0128]
[0129]
[0130] Solution preparation:
[0131] 0...
Embodiment 1
[0176] The imprecision test result of embodiment 1, comparative example 1.1 is as shown in table 2:
[0177] Table 2
[0178]
[0179] It can be seen that the imprecision of Example 1 is significantly lower than that of Comparative Example 1.1, indicating that the detection result using the scheme of Example 1 is more accurate.
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