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Immunodetection kit and measurement method and preparation method thereof

An immunodetection and kit technology, applied in the field of immunoassay kits, can solve the problems of insufficient release and inaccurate results, and achieve the effects of simple storage, simple preparation method and convenient preparation.

Pending Publication Date: 2019-12-31
DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] An object of the present invention is to provide an immunoassay kit, which includes a conjugate carrier and an immunochromatographic test strip, which can be stored and transported at room temperature, and at the same time has the characteristics of good precision, and the operation method is simple Fast, suitable for instant detection, overcomes the problem of inaccurate results caused by too much retention of conjugates on the binding pad and insufficient release in conventional test strips

Method used

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  • Immunodetection kit and measurement method and preparation method thereof
  • Immunodetection kit and measurement method and preparation method thereof
  • Immunodetection kit and measurement method and preparation method thereof

Examples

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Effect test

preparation example Construction

[0101] (Preparation method of immunoassay kit)

[0102] The method for preparing the above-mentioned immunoassay kit provided by the present invention, such as Figure 5 shown, including the following steps:

[0103] S100, preparing a conjugate carrier;

[0104] S200, preparing immunochromatographic test paper;

[0105] S300, packaged immunoassay kit.

[0106] Among them, such as Figure 6 As shown, in step S100, preparing the conjugate carrier includes:

[0107] S110, combining the ligand and the label to obtain a conjugate;

[0108] S120. Prepare a diluent, disperse the obtained conjugate in the diluent, and dilute the conjugate to a certain concentration;

[0109] S130, fixing the above diluted solution on the solid phase carrier by spraying or smearing, and drying to obtain the conjugate carrier;

[0110] S140, cutting the conjugate carrier into a fixed size, and dispensing it in a sealed chamber.

[0111] Wherein, in step S200, preparing immunochromatography test ...

Embodiment 1A

[0122] Embodiment 1AFP fluorescent microsphere immunoassay kit

[0123] 1. Structure of AFP fluorescent microsphere immunoassay kit:

[0124] It includes an immunochromatographic test strip and a conjugate solid phase carrier, the immunochromatographic test strip includes a bottom plate, and the bottom plate is sequentially connected with a first sample pad, a second sample pad, a nitrocellulose membrane and an absorbent pad, The T-line position of the nitrocellulose membrane is coated with AFP antibody, and the C-line position is coated with goat anti-mouse IgG; the label in the conjugate solid-phase carrier is europium ion fluorescent microspheres, the ligand is AFP antibody, and the solid-phase carrier is Glass fiber, the conjugate solid phase carrier is placed in a centrifuge tube.

[0125] 2. Preparation of AFP fluorescent microsphere immunoassay kit:

[0126] Reagents and consumables:

[0127] Table 1

[0128]

[0129]

[0130] Solution preparation:

[0131] 0...

Embodiment 1

[0176] The imprecision test result of embodiment 1, comparative example 1.1 is as shown in table 2:

[0177] Table 2

[0178]

[0179] It can be seen that the imprecision of Example 1 is significantly lower than that of Comparative Example 1.1, indicating that the detection result using the scheme of Example 1 is more accurate.

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Abstract

The invention provides an immunodetection kit. The immunodetection kit comprises 1) a conjugate carrier and 2) an immunochromatography test strip, wherein the conjugate carrier comprises a ligand, a marker and a solid-phase carrier, the ligand is used for generating specificity reaction with a to-be-measured analysis substance, the marker is used for signal detection, the ligand is connected withthe marker, and the solid-phase carrier is used for bearing the ligand and the marker, the conjugate carrier and the immunochromatography test strip are separately arranged. The kit can be stored andtransported under a room temperature and also has the characteristics of favorable accuracy, and the operation method is simple and rapid and is suitable for immediate detection. During measurement ofthe kit, a sample solution and the conjugate carrier are uniformly mixed and then are added into the immunochromatography test strip for detection, and a conjugate is more fully released. The kit isconvenient and rapid to fabricate, is low in manufacturing cost and can be compatible with existing assembly line equipment very well, and complicated process and high-cost equipment are not needed.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to an immunoassay kit, its assay method and preparation method. Background technique [0002] Immunochromatography is a method capable of qualitatively and quantitatively analyzing trace analytes in a short period of time using antigen-antibody reactions, and has been used to diagnose or detect many diseases and various fields, including medicine, agriculture, animal husbandry industry, food, military and environmental fields. [0003] Immunochromatographic assays are typically performed using assay strips that contain a reactive substance that changes in response to the analyte being detected, or assay devices that include assay strips housed in a plastic housing . Conventional assay test strips include: sequentially connected sample pads, conjugated pads, nitrocellulose membranes and absorbent pads, which are sequentially lapped and assembled on the base plate, wherein the sample pads...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/577G01N33/533G01N33/543
CPCG01N33/558G01N33/577G01N33/533G01N33/54313
Inventor 熊亮郑兰花洪礼清蒋奎胜庾琼刘仁源刘勇娥李建霖
Owner DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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