Griseofulvin strain solid medium

A technology of solid medium and griseofulvin, which is applied in the direction of fungi, microorganism-based methods, microorganisms, etc., can solve the problems affecting the stability of griseofulvin strains, unstable hardness, and long cultivation period, etc., to achieve The spore synchronization is good, the fermentation unit of the strain is stable, and the preparation is convenient.

Inactive Publication Date: 2020-01-10
CHIFENG PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional sucrose formula medium has a long culture cycle and unstable hardness; the potato components in the traditional PDA medium formula...

Method used

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  • Griseofulvin strain solid medium
  • Griseofulvin strain solid medium

Examples

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Embodiment 1

[0015] Embodiment 1: a kind of griseofulvin strain solid culture medium, it is formulated by the raw material of following weight ratio: 120 parts of potato starch, 20 parts of sucrose, 4 parts of potassium dihydrogen phosphate, 3 parts of potassium chloride , 1 part of sodium nitrate, 0.4 part of magnesium sulfate, 0.02 part of ferrous sulfate, 20 parts of agar, and adjust the pH to 5.5.

Embodiment 2

[0016] Embodiment 2: a kind of griseofulvin strain solid culture medium, it is formulated by the raw material of following weight ratio: 125 parts of potato starches, 25 parts of sucrose, 3 parts of potassium dihydrogen phosphate, 4 parts of potassium chloride , 2 parts of sodium nitrate, 0.5 part of magnesium sulfate, 0.01 part of ferrous sulfate, 18 parts of agar, adjust the pH to 5.3.

Embodiment 3

[0017] Embodiment 3: a kind of griseofulvin strain solid culture medium, it is formulated by the raw material of following weight ratio: 140 parts of potato starches, 30 parts of sucrose, 3.5 parts of potassium dihydrogen phosphate, 3.5 parts of potassium chloride , 1.5 parts of sodium nitrate, 0.45 parts of magnesium sulfate, 0.02 parts of ferrous sulfate, 22 parts of agar, adjust the pH to 5.7.

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Abstract

The invention discloses a griseofulvin strain solid medium. The griseofulvin strain solid medium is prepared from the following raw materials in parts by weight: 100-150 parts of potato starch, 20-30parts of sucrose, 2-4 parts of monopotassium phosphate, 3-4 parts of potassium chloride, 1-2 parts of sodium nitrate, 0.4-0.5 part of magnesium sulfate, 0.01-0.02 part of ferrous sulfate and 15-25 parts of agar. The medium is smooth in surface, moderate in hardness and uniform in inoculation; strains cultured by the medium are full in bacterial colony, white in color, round in shape, large in spore quantity, good in spore synchronization and stable in strain fermentation unit; and the medium is convenient to prepare, simple and easy to learn and high in work efficiency.

Description

technical field [0001] The invention belongs to the technical field of culture medium for strains, in particular to a solid medium for strains of griseofulvin. Background technique [0002] Griseofulvin (griseofulvin) is an aromatic derivative antibiotic and is an antifungal antibiotic. Griseofulvin is widely used in clinical treatment of skin and cuticle fungal infections; it also has a good application prospect in agricultural plant protection. The strains used in the domestic fermentation production of griseofulvin are the wild-type Penicillium patulum 4541 strains selected by my country, and the offspring N15-3-40 of D-756 strains obtained through artificial mutagenesis. [0003] At present, domestic enterprises use traditional sucrose formula medium and PDA solid medium to prepare strains for industrial production of griseofulvin. The sucrose formula medium is prepared from the following raw materials in weight percentage: 3% sucrose, 0.5% potassium dihydrogen phospha...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/04C12R1/83
CPCC12N1/14C12P17/04
Inventor 曹凤娟张国银胡建华张红玉付桂杰闫雪春丛立双
Owner CHIFENG PHARMA CO LTD
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