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A kind of compound Bacillus inoculum and preparation method and application

A technology of compounding bacillus and bacillus, applied in the field of microorganisms, can solve the problems of reduced efficacy, weakened efficacy, and reduction of beneficial bacteria in the root system, and achieve the effects of stable efficacy, easy operation, and cost saving

Active Publication Date: 2022-08-02
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most domestic compound bacteria products are cultured by single bacteria, and then mixed to obtain "complex bacteria" products. The "composite bacteria" products obtained by this method may have antagonism in themselves, resulting in weakened efficacy or reduced beneficial bacteria in the root system after application. Reduced efficacy

Method used

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  • A kind of compound Bacillus inoculum and preparation method and application
  • A kind of compound Bacillus inoculum and preparation method and application
  • A kind of compound Bacillus inoculum and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of a compound Bacillus microbial inoculum is carried out by the following steps:

[0030] (1) The preserved Bacillus methylotrophicus KNK-1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02 were inoculated into liquid LB medium respectively, and cultured at 30℃±1℃ until In the logarithmic growth period, the concentration of seed solution is not less than 1.0×10 8 cfu / mL, to obtain seed solution for inoculation;

[0031] (2) mixing the methylotrophic Bacillus Bacillus methylotrophicus KNK-1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02 obtained in step (1) to obtain a mixed seed solution, and the methyl group in the mixed seed solution The ratio of viable bacteria of Bacillus methylotrophicusKNK-1, Bacillus cereus BCJB01 and Bacillus megateriumBMJBN02 was 2:2:6;

[0032] (3) inoculate the mixed seed liquid obtained in step (2) into the fermentation medium with an inoculation amount of 10% (volume percentage), so that the initial con...

Embodiment 2

[0040] Embodiment 2 Application of compound bacillus inoculum in promoting pepper factory seedling raising and production

[0041] Proceed as follows:

[0042] (1) The compound Bacillus inoculum is added to the seedling matrix at 10 g / L, and the cultivation matrix is ​​obtained after fully mixing, sowing and routine management of "Zhongjiao No. 6" according to the conventional method, directly using the seedling matrix (the Formula: The formula of the seedling substrate produced by Pederman Company: organic matter content ≥ 45%, humic acid content ≥ 15%, PH value: 5.5 ~ 6.8, free water content ≤ 20%) "Zhongjiao No. 6" as a cultivation substrate "The cultivation group is the negative control, and the cultivation group of "Zhongjiao No. 6" which is added with nitrogen, phosphorus and potassium compound fertilizer (nitrogen: phosphorus: potassium = 15:15:15) in the cultivation substrate is the positive control (the usage amount is 60g / m2). 2 ), 30 plants were treated in each gro...

Embodiment 3

[0051] Embodiment 3 Application of compound bacillus inoculant in preventing and controlling pepper diseases (comparison of product of the present invention and common pesticides in the field)

[0052] The application of a compound bacillus inoculant in preventing and treating pepper diseases is carried out through the following steps:

[0053] (1) The test object is the seedlings of the moderately sensitive variety "Zhongjiao No. 6" with the same growth in Example 2 (1). The test was carried out in the greenhouse experimental field of the Institute of Ecology, Shandong Academy of Sciences. The experimental field had outbreaks of chili frost in 2017 and 2018. Mildew, pepper blight and pepper blight.

[0054] (2) A total of 5 treatments were tested: the seedlings of "Zhongjiao No. 6" treated with the compound Bacillus in Example 2 (1) were composed of 45% chlorothalonil 200 times liquid, 64% antiviral alum wettable powder 500 times, the compound Bacillus inoculum is 100 times ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a compound bacillus inoculant. These include Bacillus methylotrophicus KNK‑1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02. All the above strains were isolated and preserved by our laboratory. The most notable feature of the present invention is the application of the compound spore inoculant in promoting plant growth and / or preventing and controlling related plant diseases caused by phytopathogenic bacteria infection. , In addition, the average control effect on common plant diseases can reach 70%, which has great application value to the cultivation of economic plants.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a compound bacillus inoculant. Background technique [0002] Since the 21st century, the population growth has caused a sharp increase in the demand for agricultural products. In order to achieve the growth of agricultural production, the abuse of chemical fertilizers and chemical pesticides is increasing day by day. The abuse of chemical preparations reduces the sensitivity of plant pathogens to fungicides and produces drug resistance, which leads to increased agricultural production costs and reduces the benefit value of agricultural products; The food chain in turn threatens human health. In order to change this bad phenomenon, in response to the national policy of encouraging and advocating green and environmentally friendly agriculture, the research of exploring new microbial inoculants to replace or partially replace chemical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00A01G24/20A01G22/05A01G13/00C12R1/07C12R1/085C12R1/11
CPCC12N1/20A01N63/00A01G24/20A01G22/05A01G13/00
Inventor 谢雪迎张新建周红姿周方园郭书海张广志范素素赵晓燕吴晓青
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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