A kind of medicine for preventing and treating fish myxosporiosis
A technology of myxosporiosis and drugs, which is applied in the field of drugs for the prevention and treatment of fish myxosporiosis, can solve problems such as fish myxosporiasis that have not yet been seen, and achieve the effect of preventing and controlling the occurrence and application of myxosporiasis in fish Good, low-cost results
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Embodiment 1
[0016] The mature spores of Honghu iodophora were isolated and purified by percoll density gradient centrifugation, and short-term culture was carried out in M199 medium supplemented with fetal bovine serum; carvacrol was diluted with DMSO and added to the medium, and the drug concentration was configured at 0, 0.625, 0.125, and 0.500 µL / mL of each test group, cultured at 25°C for 48 hours, stained with SYTOX green nucleic acid stain fluorescent dye, and counted the survival rate of spores of Honghu iodine under a fluorescent microscope. The test results showed that the kill rates of the drug-added groups were about 0, 35%, 63%, and 86%. The spore killing effect is counted under the microscope, see the appendix figure 1 and 2 ; figure 1 It is a picture of the spores of Honghu iodine under the microscope, figure 2 It is the killing effect of Honghu iodine spores under the fluorescence microscope, only the killed spores can show fluorescence.
Embodiment 2
[0018] Based on crucian carp commercial feed (total fat 7%, total protein 30%), add 0, 0.05%, 0.1%, 0.2%, 0.4%, 0.8% carvacrol respectively; healthy heterogeneous gibel crucian carp seedlings (30-50g) was purchased from a disease-free crucian carp fry breeding farm, randomly set up 6 groups, 20 fish in each group, fed the test fish with 2.5% bait every day, observed and recorded the death of fish for 96 hours, and evaluated the effect of drugs on heterogeneous silver. crucian carp safety. The test results show that there is no death in each drug concentration group, and carvacrol is safe to crucian carp at the test concentration.
Embodiment 3
[0020] Put 5,000 heterogeneous gibel seedlings (50-70g) purchased from the epidemic area in an outdoor 2-acre breeding pond, randomly sample 50, dissect false gills, ovaries, and kidneys, and extract tissue genome DNA with a commercial kit , The specific PCR detection method of samples from Honghu Lake confirmed that 40% of stocked fish species were infected with Honghu iodine. During the breeding water temperature of 25-28°C, feed carvacrol medicinal bait (add 0.1% carvacrol to crucian carp commercial feed) for 3 days, and repeat feeding medicinal bait once after 2 weeks. In the whole breeding period (8 weeks), the heterogeneous gibel carp in ponds did not have Honghu iodine efferocystosis.
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