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Molecular marker closely linked with brilliant green properties of brassica campestris stems and application of molecular marker

A molecular marker, head cabbage technology, applied in the field of molecular biology, can solve the problems of double-gene recessive inheritance, single-gene recessive inheritance, incomplete dominant inheritance, etc., and achieves the effect of reducing blindness and good specificity

Active Publication Date: 2020-01-24
荆州农业科学院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the genetic and genetic research on wax powder traits in Brassica plants is very extensive, but there are differences in their inheritance modes, including single-gene recessive inheritance, single-gene dominant inheritance, double-gene recessive inheritance and incomplete dominance. heredity

Method used

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  • Molecular marker closely linked with brilliant green properties of brassica campestris stems and application of molecular marker
  • Molecular marker closely linked with brilliant green properties of brassica campestris stems and application of molecular marker
  • Molecular marker closely linked with brilliant green properties of brassica campestris stems and application of molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Development and application of molecular markers linked to bright green traits of non-heading cabbage stems:

[0020] 1. Selection of parents

[0021] The parental material Q195 is a multi-generation inbred line with wax powder on the stem, gray-green wax powder on the stem, late bolting period, thick stem, large leaves, and large plant expansion; the parent material Q161 is bright green on the stem Inbred line of the first generation, the stems are bright green without obvious wax powder, the bolting stage is early, the stems are thinner, the leaves are smaller, the plant development is smaller, and the growth of the plants is weaker than that of the wax powder materials.

[0022] 2. Parent and F 1 , F 2 、BC 1 P 1 、BC 1 P 2 Field phenotyping of offspring

[0023] In the spring of 2018, Q195 and Q161 were planted and hybridized to obtain F 1 Generation hybrids, F 1 F 2 、BC 1 P 1 、BC 1 P 2 In the autumn of 2018, the parents and the population were planted i...

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Abstract

The invention discloses a molecular marker closely linked with brilliant green properties of brassica campestris stems and an application of the molecular marker. 6 InDel markers co-separated from brilliant green genes of brassica campestris stems are obtained through screening through a BSA genome re-sequencing technique and an InDel marking technique, the molecular marker can quickly breed the brassica campestris having brilliant green stems and assist breeding of brassica campestris new species, and requirements of agricultural production can be well met.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a molecular marker closely linked to the bright green character of non-heading cabbage stalks and an application thereof. Background technique [0002] Plant wax is composed of long-chain saturated fatty acids, alcohols, alkanes, aldehydes, ketones and other substances. Waxy crystals often exist in the form of rods, filaments, tubes, discs, etc. in stems, leaves, seed pods and On the epidermis of the style. The epidermis of many plants is covered with a layer of wax, and its wax composition and content will vary due to different species and their organs, and the wax content of the same organ of the same plant will also vary due to different environments. The waxy layer of plant epidermis plays an important role in plant growth and development, including reducing non-stomatal water loss, resisting the invasion of some pathogens, and resisting the encroachment of some ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 胡阳宋莉萍汪爱华李平高长斌梁红艳周国林崔晓培邱文兵蔡美仲
Owner 荆州农业科学院