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A method for promoting the accumulation of nutrients in tissue seedlings of mulberry

A nutrient substance and tree-building technology, which is applied in the fields of biotechnology and agricultural engineering, can solve problems such as low multiplication coefficient, low bioenergy efficiency, and difficult rooting, and achieve high nutrient content, remarkable energy-saving effect, and high reproduction coefficient.

Active Publication Date: 2021-08-24
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficiency of conventional sowing and cutting propagation of mulberry is low. With the surge in demand for seedlings in the market, tissue culture and rapid propagation have become the main way to obtain high-quality mulberry seedlings
At present, the research on tissue culture and rapid propagation of mulberry tree is mostly focused on the selection of culture conditions such as material sites, medium types, hormone levels, etc., but usually white fluorescent lamps are used as light sources, because their spectral energy distribution does not meet the needs of plants, and the bioenergy efficiency is extremely high. Low and there are problems such as low multiplication coefficient and difficult rooting

Method used

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  • A method for promoting the accumulation of nutrients in tissue seedlings of mulberry
  • A method for promoting the accumulation of nutrients in tissue seedlings of mulberry
  • A method for promoting the accumulation of nutrients in tissue seedlings of mulberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Basic medium configuration:

[0035] The basic medium is MS medium, in which the components of major elements and their corresponding concentrations are as follows; KNO 3 1900mg / L, NH 4 NO 3 1650mg / L, KH 2 PO 4 170mg / L, MgSO 4 ·7H 2 O 370mg / L, CaCl 2 2H 2 O 440mg / L;

[0036] The components of trace elements and their corresponding concentrations are as follows: KI 0.83mg / L, H 3 BO 3 6.2mg / L, MnSO 4 ·H 2 O22.3mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, Na 2 MoO4 2H 2 O 0.25mg / L, CuSO 4 ·5H 2 O 0.025mg / L, CoCl 2 .6H 2 O 0.025mg / L;

[0037] The composition of iron salt and its corresponding use concentration are as follows: Na 2 EDTA 37.3mg / L, FeSO 4 ·7H 2 O27.8mg / L;

[0038] The components of organic ingredients and their corresponding concentrations are as follows: inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride VB 1 0.1mg / L, pyridoxine hydrochloride VB 6 0.5mg / L, Niacin VB 5 0.5mg / L.

[0039] The induction medium and the proliferation (rootin...

Embodiment 2

[0050] Basic medium configuration:

[0051] The basic medium is MS medium, in which the components of major elements and their corresponding concentrations are as follows; KNO 3 1900mg / L, NH 4 NO 3 1650mg / L, KH 2 PO 4 170mg / L, MgSO 4 ·7H 2 O 370mg / L, CaCl 2 2H 2 O 440mg / L;

[0052] The components of trace elements and their corresponding concentrations are as follows: KI 0.83mg / L, H 3 BO 3 6.2mg / L, MnSO 4 ·H 2 O22.3mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CuSO 4 ·5H 2 O 0.025mg / L, CoCl 2 .6H 2 O 0.025mg / L;

[0053] The composition of iron salt and its corresponding use concentration are as follows: Na 2 EDTA 37.3mg / L, FeSO 4 ·7H 2 O27.8mg / L;

[0054] The components of organic ingredients and their corresponding concentrations are as follows: inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride VB 1 0.1mg / L, pyridoxine hydrochloride VB 6 0.5mg / L, Niacin VB 5 0.5mg / L.

[0055] The induction medium and the proliferation (root...

Embodiment 3

[0066] Basic medium configuration:

[0067] The basic medium is MS medium, in which the components of major elements and their corresponding concentrations are as follows; KNO 3 1900mg / L, NH 4 NO 3 1650mg / L, KH 2 PO 4 170mg / L, MgSO 4 ·7H 2 O 370mg / L, CaCl 2 2H 2 O 440mg / L;

[0068] The components of trace elements and their corresponding concentrations are as follows: KI 0.83mg / L, H 3 BO 3 6.2mg / L, MnSO 4 ·H 2 O22.3mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CuSO 4 ·5H 2O 0.025mg / L, CoCl 2 .6H 2 O 0.025mg / L;

[0069] The composition of iron salt and its corresponding use concentration are as follows: Na 2 EDTA 37.3mg / L, FeSO 4 ·7H 2 O27.8mg / L;

[0070] The components of organic ingredients and their corresponding concentrations are as follows: inositol 100mg / L, glycine 2mg / L, thiamine hydrochloride VB 1 0.1mg / L, pyridoxine hydrochloride VB 6 0.5mg / L, Niacin VB 5 0.5mg / L.

[0071] The induction medium and the proliferation (rooti...

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Abstract

The invention relates to a method for promoting the accumulation of nutrients in paper mulberry tissue seedlings. The tender stems of paper mulberry are used as explants, and a special LED light source and a multiplying light formula are used to successfully establish a paper mulberry tissue culture high-efficiency regeneration system. Tree factory seedlings provide a reference technology and method, and at the same time lay the foundation for the development of factory seedlings. By adopting the tissue culture method of the present invention, the proliferation multiple of the paper paper hybrid clone in 30 days reaches 6 to 8 times, compared with the traditional light source culture method, the proliferation multiple is increased by 2 to 3 times, and the crude protein content in the leaves is increased by 85% to 90%. %, the total phosphorus content increased by 100% to 130%, the total potassium content increased by 100% to 128%, and the cellulose decreased by 8% to 15%, which improved the nutrient content and feed quality of leaves.

Description

technical field [0001] The invention relates to the fields of biotechnology and agricultural engineering, in particular to a method for promoting the accumulation of nutrient substances in tree tissue seedlings. Background technique [0002] Broussonetia papyrifera (L.) vent is a deciduous tree belonging to the genus Broussonetia of the family Moraceae. The mulberry tree has strong adaptability, wide distribution, resistance to drought and barrenness, and salt-alkali tolerance. Well, livestock and poultry like to eat. Conventional sowing and cutting propagation of mulberry are inefficient. With the surge in demand for seedlings in the market, tissue culture and rapid propagation have become the main way to obtain high-quality mulberry seedlings. At present, the research on tissue culture and rapid propagation of mulberry tree is mostly focused on the selection of culture conditions such as material sites, medium types, hormone levels, etc., but usually white fluorescent la...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄婧陈庆生张敏周鹏蒋泽平
Owner JIANGSU ACAD OF FORESTRY
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