Quality control method for dish culture of antrodia camphorata

Abstract

The invention relates to a quality control method for dish culture of antrodia camphorate, and relates to a triterpene antrodia camphorata acid compound. The quality control method comprises the stepsof: preparing test sample solution; preparing a reference substance; establishing test sample HPLC fingerprint spectrum conditions; inspecting system adaptability; inspecting content of a pair of main content epimer compounds; and confirming method accuracy by an external standard method. By the provided preparation method of the reference substance, the reference substance with purity of over 98% is obtained. Baseline separation is implemented by a test sample HPLC fingerprint spectrum and one pair of main content epimers. Test sample preparation repeatability is high. The prepared test sample and reference substance solution is high in stability and can be used for content measurement. The three inspected compounds in a quality range of 0.1 to 4.8 mu g have a good linear method relationship, and a correlation coefficient is greater than 0.9999. The quality control method is high in accuracy and a sampling recovery rate of the quality control method is within a range of 98% to 104%.Quality control of dish culture of the antrodia camphorate is researched, and problems that a great of amount of reference substances are difficult to obtain and content measurement cannot be accurately carried out are solved.

Description

technical field [0001] The invention relates to triterpenoid antrodia acid compounds, in particular to a quality control method for cultured Antrodia camphorata in a dish culture. Background technique [0002] Due to a series of reasons such as the shortage of wild Antrodia camphorata host Cinnamomum camphora resources and the long cultivation period, the yield of wild Antrodia camphorata is too small, so it is necessary to increase the research on artificially cultivated Antrodia camphorata. After comprehensive consideration of various culture methods, dish culture culture Antrodia camphorata has good market application value because of its simple culture method, moderate culture period and no need to rely on host Cinnamomum camphora. Moreover, both cultured Antrodia camphorata and wild Antrodia camphorata contain a large amount of active ingredients of antrodia acids, which also have the same effect on pharmacological activity, which provides a basis for the market applica...

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Problems solved by technology

[0003] However, since there are many epimers in these triterpenes, for example, the main components (25S)-Antcin K and (25R)-Antcin K are a pair of epimers. In the study of Antrodia cinnamomea fruiting bodies harvested at different culture ages and from different wood substrates[J].JAgric Food Chem,2011,59(14):7626-35), and the quantitative components did not reach the baseline separation in the HPLC fingerprint ([2]Y-M T.Method for HPLC analysis of tritepenoids from antrodia campphora[J])

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