A microfluidic sampling system

Abstract

The invention discloses a microfluidic sampling system, which includes a quantitative sampling module, an air pressure drive and control module, and a negative pressure generator; the quantitative sampling module includes a sample pool, an inlet working channel, a first one-way valve, a first hydrophobic Valve, quantitative sampling chamber, second steam trap, third steam trap, second one-way valve, inlet working channel; the first one-way valve is set on the inlet working channel; the second one-way valve is set on the outlet working channel The inlet and outlet ports of the quantitative sampling chamber are respectively the first steam trap and the second steam trap; a third steam trap is arranged between the second steam trap and the second one-way valve; the pneumatic drive and control module includes a micro air pump, The miniature solenoid valve and the controller, the air pump is the air source to provide the driving pressure, the solenoid valve and the controller form the control unit to control the on-off of the driving pressure. The system can improve the precision of quantitative sample injection, and can control the flow rate and flow rate of the sample according to the principle of pulse modulation during sample injection, and can save the cost of sample injection.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, in particular to a microfluidic sampling system. Background technique [0002] Microfluidics is an interdisciplinary science and technology involving fluid mechanics, precision machinery, biomedicine, chemistry, etc. It is widely used in biochemical analysis, medical diagnosis, drug screening, etc. Due to the advantages of fewer samples / reagents and convenient miniaturization of instruments and equipment, it has achieved rapid development in recent years. Fluid sampling is a key link in the engineering application of microfluidic technology, involving quantitative sampling, fluid drive and sampling flow rate control, etc. This operation can be accomplished with a built-in integrated micropump, but the structure is complex and costly, and it is not suitable for disposable microfluidic chips. If the sample feeding methods such as external syringe pump and pressure pump are used, it is easil...

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Problems solved by technology

For example Jessica Melin&Stephen R.Quake, Microfluidic Large-ScaleIntegration: The Evolution of Design Rules for Biological Automation (J), Annu.Rev.Biomol.Struct.2007.36:213-231. The method of controlling the opening and closing of the control channel to complete the quantitative sampling of micro samples. In order to realize online quantitative sampling, this method usually requires multiple control microvalves, the structure is relatively complicated, and the flow dead zone in the microvalve is likely to cause liquid residue, which affects the sampling accuracy.

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