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a detection hg 2+ Preparation method of self-enhanced electrochemiluminescence aptamer sensor

An aptamer sensor, electrochemical technology, applied in chemiluminescence/bioluminescence, material electrochemical variables, scientific instruments, etc., can solve the problems of expensive instruments, relying on professionals, etc., to achieve simple preparation methods, simplification of complexity, selective effect

Active Publication Date: 2022-02-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods are accurate and efficient, they rely on professionals and expensive instruments

Method used

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  • a detection hg <sup>2+</sup> Preparation method of self-enhanced electrochemiluminescence aptamer sensor
  • a detection hg <sup>2+</sup> Preparation method of self-enhanced electrochemiluminescence aptamer sensor
  • a detection hg <sup>2+</sup> Preparation method of self-enhanced electrochemiluminescence aptamer sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] according to figure 1 Described preparation process:

[0045] (1) apt-NGQDs-NH 2 -Ru@SiO 2 Preparation of composite materials:

[0046] First, mix 1.77mL triton, 7.5mL cyclohexane, 1.8mL 1-hexanol and 340μL secondary water and stir together for 15min, then add 80μL ruthenium terpyridyl, 100μL tetraethyl silicate, 60μL ammonia water, and continue stirring The time is 24 hours. After the stirring, centrifuge with acetone and ethanol, and wash with water three times each, at a centrifugation speed of 10,000 rpm, for 5 minutes each time. Finally, the precipitate obtained is dissolved in ultrapure water and set aside.

[0047] The Ru@SiO obtained above 2 (2mg / mL) 4mL and 1600μL APTES were mixed and stirred for 4h. After the reaction, the obtained product was dissolved in ethanol and centrifuged three times at 10000rpm for 5min each time. 2 -Ru@SiO 2 .

[0048] Will NH 2 -Ru@SiO 2 (1mg / mL) 1mL and NGQDs (5mg / mL) 5mL were mixed and stirred for 12h to obtain a yellow s...

Embodiment 2

[0057] The prepared self-enhanced electrochemiluminescence aptasensor for detection of Hg 2+ Optimize experimental conditions:

[0058] The biosensor prepared in Example 1 was placed at 4° C. for 6, 8, 10, 12, and 14 hours to detect ECL signals.

[0059] figure 2 (A) for apt-NGQDs-NH 2 -Ru@SiO 2 The relationship between incubation time and ECL; the incubation time increased from 4h to 8h, and the ECL signal intensity gradually increased and reached a plateau at 8h. Therefore, 10h was chosen as the optimal incubation time for aptamers.

Embodiment 3

[0061] The prepared self-enhanced electrochemiluminescence aptasensor for detection of Hg 2+ Optimize experimental conditions:

[0062] On the basis of the self-enhancing electrochemiluminescence aptasensor with the best conditions obtained in Example 2, modify the target Hg on the surface of the sensor 2+ ,Such as figure 2 (B) is the relationship between the incubation time of the target and the ECL. The incubation time increased from 40min to 70min, and the ECL signal intensity gradually increased, reaching a plateau at 80min, and the Hg of the target 2+ The incubation time on the electrode surface is 90min.

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Abstract

The invention belongs to the technical field of biosensors, and relates to a method for detecting Hg 2+ Preparation method of self-enhanced electrochemiluminescence aptasensor. Self-enhanced electrochemiluminescence is different from traditional electrochemiluminescence. The emitter and co-reactant are combined in one molecule. In order to further improve the selectivity, the aptamer is combined with the luminescent group, and the T-Hg 2+ The characteristic structure of ‑T was first applied to the Hg 2+ detection. In the present invention, the introduction of Hg 2+ Aptamers not only improve the sensor's Hg 2+ Highly specific, and on the basis of self-reinforcement, by T‑Hg 2+ ‑T structure, the aptamer is bent, so that the self-reinforcement material connected at one end of the aptamer is close to the electrode, the electron transfer distance is shortened, and the ECL luminous efficiency is further improved. The constructed sensor detection linear range is 5.0×10 ‑11 -1.0×10 ‑6 M, the detection limit is 1.0×10 ‑12 M. Through exploration, the preparation method of the sensor is simple, with high selectivity, high sensitivity, good reproducibility, and good stability. 2+ Provides a good sensing platform.

Description

technical field [0001] The invention belongs to the technical field of biosensing detection, and relates to a method for detecting Hg 2+ The preparation method specifically relates to a kind of based on apt-NGQDs-NH 2 -Ru@SiO 2 The preparation method and application of the self-enhanced electrochemiluminescence aptasensor of the system. Background technique [0002] Mercury ion (Hg 2+ ) is a heavy metal ion with high toxicity and easy accumulation. Hg 2+ It is widely distributed in soil, crops, water, and the atmosphere, and enters the human body through the biological chain. When the ecological environment Hg 2+ If the content is too high, it will lead to soil methylation, plant poisoning, water pollution, and damage to aquatic organisms such as fish and shrimp. After entering the human body through the food chain, it will be enriched in the body, causing deformation of limbs, and even the terrible Minamata disease. Therefore for Hg in water 2+ It is necessary to de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/38G01N21/76
CPCG01N27/3275G01N27/3278G01N27/308G01N27/38G01N27/305G01N21/76
Inventor 由天艳陈柏年李丽波刘晓红毕晓雅
Owner JIANGSU UNIV
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