Method for removal of impurities from bacterial capsular polysaccharide based preparations

A technology of polysaccharide and polysaccharide solution is applied in the directions of antibacterial drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve the problems of time-consuming, expensive, and complicated process.

Active Publication Date: 2020-02-21
SERUM INST OF INDIA PTE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, the steady supply of DOC with the required regulatory certifications is limited to a single vendor / supplier
[0028] The high consumption of ethanol and the addition of a redundant step (carbon filtration) make the process cumbersome, time-consuming and expensive

Method used

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  • Method for removal of impurities from bacterial capsular polysaccharide based preparations
  • Method for removal of impurities from bacterial capsular polysaccharide based preparations
  • Method for removal of impurities from bacterial capsular polysaccharide based preparations

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Experimental program
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Embodiment approach

[0043] According to a first embodiment of the invention, centrifugation is used to separate and purify bacterial capsular polysaccharides from the inactivated harvest. The supernatant was diafiltered with a 100 kD tangential flow filtration unit. It is very clear to a person skilled in the art that instead of centrifugation and diafiltration any other suitable method can be used to concentrate bacterial capsular polysaccharides. In one of the preferred aspects of this embodiment, the capsular polysaccharide is derived from Neisseria meningitidis serogroups A, C, W, Y and X.

[0044] According to a second embodiment of the invention, the retentate obtained in the first embodiment is treated with an anionic surfactant / detergent. Anionic detergents are selected from the group comprising alkyl sulphates, sodium lauryl sulphate, sodium deoxycholate, sodium dodecyl sulphate, sodium s-alkyl sulphate , fatty alcohol polyoxyethylene ether sodium sulfate, sodium oleyl sulfate, N-oleyl...

no. 3 approach

[0047] According to a third embodiment of the present invention, a strong base is added to the mixture obtained in the above embodiment, and the pH of the strong base is adjusted to be between pH9 and pH11 with continuous stirring at room temperature for 1 hour. The strong base is selected from the group comprising sodium hydroxide, potassium hydroxide, sodium carbonate, hydroxylamine, triethylamine and lithium hydroxide.

[0048] According to a preferred aspect of the third embodiment of the present invention, the strong base, i.e. sodium hydroxide, with a final concentration between 5M and 20M is added to the mixture obtained by the above embodiment, and the mixture is stirred at room temperature for 1 hour to dissolve the The pH of the strong base, sodium hydroxide, was adjusted to pH 10.5.

[0049] In another aspect of the third embodiment of the present invention, instead of adding base, EDTA and sodium acetate are added to the mixture obtained from the second embodiment,...

no. 10 approach

[0057] According to the tenth embodiment of the present invention, the solution obtained from the above embodiment is fully diafiltered through WFI (Water for Injection) using 100 kDa tangential flow filtration, and passed through a 0.2μ filter, and heated at -20°C or -20 ℃ below as the final bulk storage.

[0058] According to the eleventh embodiment of the present invention, the purified capsular polysaccharides of Neisseria meningitidis serogroups C, W and Y are obtained according to the following steps:

[0059] • N. meningitidis serogroups C, W and Y were grown separately in appropriate media and inactivated using formaldehyde.

[0060] • Add sodium lauryl sulfate to the inactivated harvest to a final concentration of 1%, and stir at room temperature for 2 hours.

[0061] • Add sodium hydroxide until the final concentration is 05mM-20mM, and adjust the pH to pH 10.5 with constant stirring at room temperature for 1 hour.

[0062] - Neutralize the solution obtained from t...

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Abstract

The present invention relates to an improved process for purification of bacterial capsular polysaccharides, more specifically capsular polysaccharides of gram negative bacteria. The process comprisesof concentration and dia filtration of harvest, treatment with anionic detergent and strong alkali followed by centrifugation, diafiltration and cationic detergent based precipitation of bacterial polysaccharides. The process results in significant reduction of endotoxin, protein and nucleic acid impurities thereby providing higher recovery of capsular polysaccharide with the desired O-acetyl levels. Said process is scalable, non-enzymatic, and employs fewer purification steps.

Description

technical field [0001] The present invention is broadly in the field of biotechnology, and in particular relates to harvesting and downstream processing for the purification of bacterial capsular polysaccharides. Background technique [0002] Neisseria meningitidis is the leading cause of infection of the membranes surrounding the brain and spinal cord (meninges) and cerebrospinal fluid (CSF), leading to death and disability worldwide. [0003] Neisseria meningitidis, also known as meningococcus, is a Gram-negative bacterium. Based on the structure of the polysaccharide capsule, 13 serogroups of N. meningitidis have been identified, of which 6 serogroups (A, B, C, W, X, and Y) have epidemic potential (ref: Lee Harrison et al., 2011). [0004] Meningitis occurs in small clusters around the world, with seasonal epidemics due to varying proportions of epidemic bacterial meningitis. The greatest burden of meningococcal disease occurs on the African continent, especially in su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C08L5/00C08H1/00A61K39/095
CPCA61K39/095A61K2039/6037A61P31/04C08B37/00C08B37/0003C08H1/00C08L5/00C12P19/04
Inventor 拉杰夫·马拉萨坎特·迪希尔桑巴吉·尚卡尔·皮萨尔塔特瑞亚·萨尔马·安南拉留
Owner SERUM INST OF INDIA PTE LTD
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