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Fluorescence brightness determination method and device and computer storage medium

A technology of fluorescence brightness and determination method, applied in the field of fluorescence signal detection, can solve the problems of high requirements and difficulty in obtaining absolute fluorescence brightness, etc.

Active Publication Date: 2020-02-28
BEIJING LINJIN SPACE AIRCRAFT SYST ENG INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the absolute fluorescence brightness of the dyed object is analyzed. Although the absolute fluorescence brightness is an important medical clue and has important clinical significance, it is difficult and demanding to obtain the absolute fluorescence brightness.

Method used

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  • Fluorescence brightness determination method and device and computer storage medium
  • Fluorescence brightness determination method and device and computer storage medium
  • Fluorescence brightness determination method and device and computer storage medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] figure 1 A schematic flow chart showing the implementation of the method for determining fluorescence brightness in Embodiment 1 of the present application is shown.

[0036] As shown in the figure, the method for determining the fluorescence brightness includes:

[0037] Step 101, collecting the first image with the object under test when the light source is turned on under the target channel;

[0038] Step 102, calculating the brightness value of the first image;

[0039] Step 103, according to the brightness value of the first image, the brightness value of the second image obtained in advance, the brightness weight of the target channel, and the brightness gain weight of the light source, calculate relative brightness values ​​of different channels; wherein, The second image is an image without the measured object when the light source is turned on under the target channel;

[0040] Step 104. Determine the fluorescence brightness of the object under test accordin...

Embodiment 2

[0104] Based on the same inventive concept, an embodiment of the present application provides a device for determining fluorescence brightness. The principle of the device for solving technical problems is similar to that of a method for determining fluorescence brightness, and repeated descriptions will not be repeated here.

[0105] figure 2 A schematic structural diagram of the device for determining fluorescence brightness in Embodiment 2 of the present application is shown.

[0106] As shown in the figure, the fluorescence brightness determination device includes:

[0107] The collection module 201 is used to collect the first image of the measured object when the light source is turned on under the target channel;

[0108] A first calculation module 202, configured to calculate the brightness value of the first image;

[0109] The second calculation module 203 is configured to calculate the brightness of different channels according to the brightness value of the firs...

Embodiment 3

[0130] Based on the same inventive concept, an embodiment of the present application further provides a computer storage medium, which will be described below.

[0131] The computer storage medium stores a computer program thereon, and when the computer program is executed by a processor, the steps of the method for determining fluorescence brightness as described in Embodiment 1 are realized.

[0132] The computer storage medium provided in the embodiment of the present application provides a fluorescence brightness evaluation method that can collect images for multi-channel objects to be tested in a fluorescent microscope, which is simple, operable, and can meet the fluorescence brightness evaluation requirements of fluorescent objects to be tested .

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Abstract

The invention discloses a fluorescence brightness determination method and device and a computer storage medium. The method comprises the steps: collecting a first image of a measured object when a light source is in an on state in a target channel; calculating a brightness value of the first image; performing calculating to obtain brightness relative values of different channels according to thebrightness value of the first image, a pre-calculated brightness value of a second image, the brightness weight of the target channel and a brightness gain weight of a light source, wherein the secondimage is an image without the measured object when a light source is in an on state under the target channel; and determining the fluorescence brightness of the measured object according to the brightness relative values of the different channels. By means of the scheme, the fluorescence brightness evaluation of the measured object can be achieved, and the method is simple and high in operability.

Description

technical field [0001] The present application relates to fluorescence signal detection technology, in particular, to a method, device and computer storage medium for determining fluorescence brightness. Background technique [0002] Immunofluorescence technique (Immunofluorescence technique), also known as fluorescent antibody technology, is one of the earliest developments in labeling immune technology, and it is a technology established on the basis of immunology, biochemistry and microscope technology. Immunofluorescence analysis is to measure the intensity of light emitted by the substance itself after the substance absorbs light of a certain frequency. Since there is a proportional relationship between the fluorescence intensity and the quantity of the substance, the substance can be qualitatively or quantitatively determined by measuring the fluorescence spectrum and fluorescence intensity. Quantitative analysis. Compared with radioimmunoassay, immunofluorescence ana...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G06T7/90
CPCG01N21/6428G01N21/6458G06T7/90G01N2021/6439G06T2207/30024
Inventor 孙宝亮商学谦张志龙郭灵犀潘红九雷净马鸣底亚峰杨飞李萌萌
Owner BEIJING LINJIN SPACE AIRCRAFT SYST ENG INST