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A kind of method that utilizes salicylic acid to promote Chinese fir somatic embryogenesis

A technology of salicylic acid and Chinese fir, which is applied in horticultural methods, botanical equipment and methods, applications, etc., to achieve the effects of good practicability, improved induction efficiency, and accelerated industrialization development

Active Publication Date: 2021-06-18
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that salicylic acid has different effects on somatic embryogenesis of different plants, and the mechanism needs to be further explored, but there is no report on its application in somatic embryogenesis of Chinese fir.

Method used

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  • A kind of method that utilizes salicylic acid to promote Chinese fir somatic embryogenesis
  • A kind of method that utilizes salicylic acid to promote Chinese fir somatic embryogenesis
  • A kind of method that utilizes salicylic acid to promote Chinese fir somatic embryogenesis

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Embodiment 1

[0022] Preparation of mother liquor: salicylic acid is easily soluble in water, weigh 1.0g of salicylic acid powder, dissolve it in a beaker with ultrapure water, and set the volume to 1L, and prepare 1mg / mL salicylic acid mother liquor, and finally transfer it to Store in a brown bottle at 4°C for later use.

[0023] Embryogenic pedicle induction and proliferation medium: Chinese fir embryogenic pedicle induction is the process of inducing callus formation in Chinese fir seeds. The medium formula is DCR medium with 0.5mg / L KT, 0.5mg / L BA, 1mg / L VC, 1~2mg / L 2,4-D, 0.45g / L glutamine, 0.5g / L hydrolyzed casein, 2.5g / L Ac, 2.3g / L crystal agar.

[0024] Somatic embryo induction medium: Chinese fir somatic embryo induction is the process of forming somatic embryos from Chinese fir callus. The medium formula is DCR basic medium with 1mg / L VC, 0.45g / L glutamine, 0.5g / L hydrolyzed casein, 2-4g / L Ac, 2.8g / L crystal agar, 0.2g / L Asparagine, 0.2g / L proline. 100-200g / L PEG, 1-5mg / L GA,...

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Abstract

The invention discloses a method for promoting somatic embryogenesis of Chinese fir by using salicylic acid, which belongs to the technical field of plant tissue culture. The method provided by the present invention is specifically as follows: DCR is used as a basic medium, and salicylic acid with a concentration of no more than 8 mg / L is added as a Chinese fir body embryo induction medium; immature seed embryos are obtained through immature cones of Chinese fir, and the Put it on the callus induction medium and cultivate it for 25 to 30 days to obtain the embryogenic stalk of Chinese fir; put the obtained Chinese fir embryogenic embryonic stalk into the somatic embryo induction medium of Chinese fir, and carry out dark culture in the incubator to induce and promote Chinese fir somatic embryogenesis, cultured for 60-100 days at 23°C, can obtain somatic embryos up to twice the number of the control, which greatly improves the induction efficiency of Chinese fir somatic embryos and has good practicability.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for promoting somatic embryogenesis of Chinese fir by using salicylic acid. Background technique [0002] Chinese fir (Cunninghina lanceolata) belongs to the fir genus of the family Fir, and is one of the important timber tree species in my country. It grows fast, has a straight and round stem shape, is light, soft and delicate in texture, easy to process, beautiful in texture, has a special aroma, and is neither warped nor cracked. Moth-resistant and corrosion-resistant, it is an excellent raw material for industries such as construction, furniture, bridges, shipbuilding, papermaking, and wood fiber. At present, there are mainly three ways to propagate Chinese fir seedlings: seeds, cuttings and traditional tissue culture [Wen Yafeng. Research on the induction and subculture of Chinese fir embryonic tissue based on immature zygotic embryos [D]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/006
Inventor 陈金慧陆叶郭玉琳施季森黄世清成铁龙胡向阳
Owner NANJING FORESTRY UNIV