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Method for increasing saponin yield of chrysanthemum leaf yams through natural fermentation

A technology of natural fermentation and chrysanthemum leaf Dioscorea, applied in directions such as fermentation, can solve problems such as low hydrolysis yield, restrict diosgenin production, pollution, etc., achieve good application prospects, improve hydrolysis yield and yield, and increase yield Effect

Inactive Publication Date: 2020-03-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the current hydrolysis production of diosgenin from Chrysanthemum leaf is faced with the problems of low hydrolysis yield and serious pollution, which limit the production of diosgenin

Method used

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  • Method for increasing saponin yield of chrysanthemum leaf yams through natural fermentation
  • Method for increasing saponin yield of chrysanthemum leaf yams through natural fermentation
  • Method for increasing saponin yield of chrysanthemum leaf yams through natural fermentation

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Embodiment 1

[0027] The natural fermentation of embodiment 1 chrysanthemum leaf yam

[0028] One, the natural fermentation method of yam chrysanthemum leaf

[0029] Accurately weigh a certain mass of Dioscorea chrysanthemum powder, put it in an oven at 105°C overnight, then weigh it, and measure the moisture content of the freeze-dried powder of Dioscorea chrysanthemum;

[0030] Weigh 10.0g (DW) of diosminum chrysanthemum freeze-dried powder, carry out fermentation for a specific time at a certain temperature according to a certain material-to-liquid ratio, that is, place it in a constant temperature incubator for cultivation, and perform hydrolysis according to the hydrolysis method of diosgenin after fermentation and extract diosgenin.

[0031] Specifically, the hydrolysis method of diosgenin is as follows: directly add concentrated sulfuric acid so that the solid-liquid ratio is 1:6, the sulfuric acid concentration is 1.0 mol / L; the hydrolysis temperature is 120° C., and the hydrolysis...

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Abstract

The invention discloses a method for increasing saponin yield of chrysanthemum leaf yams through natural fermentation. The method comprises the steps of performing natural fermentation on chrysanthemum leaf yams, and then performing acid hydrolysis; after an acid hydrolysis reaction is completed, performing cooling to room temperature, and then performing neutralization, filtration and drying so as to obtain hydrolysate; and performing extraction on the hydrolysate with petroleum ether to obtain diosgenin. According to the method disclosed by the invention, the chrysanthemum leaf yams are subjected to natural fermentation and acid hydrolysis, so that compared with the yield of the saponin obtained in a manner of directly performing hydrolysis on the diosgenin, the yield of the saponin obtained by the method is increased by 10%, and the hydrolysis yield and the production rate of the diosgenin are greatly increased. The preparation method disclosed by the invention has good applicationprospects and is worth wide popularization.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, and more specifically relates to a method for increasing saponin yield by natural fermentation of Dioscorea chrysanthemi. Background technique [0002] Dioscorea chrysanthemum is a plant of the genus Dioscorea in the Dioscoreaceae family. Originally produced in Mexico, it was successfully introduced to China's Yunnan Province in the 1970s for trial planting, and is currently widely planted in my country. Chrysanthemum leaf diosgenin content is higher, and its saponin content is twice that of the same weight of diosminum scutellariae. It is an important raw material for extracting saponin and synthesizing other corticosteroids. [0003] The steroidal structure of diosgenin is simple and easy to chemically modify. It is the main raw material for the synthesis of steroid hormone drugs, the second largest drug after antibiotics. It is called medical gold and has a high market value. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20
CPCC12P33/20
Inventor 谢君叶广英毕桂灿
Owner SOUTH CHINA AGRI UNIV
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