A kind of method for obtaining leek male sterile plant

A technology of male sterility and chives, applied in the field of vegetable genetics and breeding, can solve the problems of no major progress and achieve the effect of increasing the rate of sterile plants

Active Publication Date: 2021-08-13
廊坊市农林科学院
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, Chinese chive breeders are paying more and more attention to the selection of male sterile lines of Chinese chives, but the sources of their male sterile lines of Chinese chives are all naturally produced in the field, and there is no great progress in the aspect of actively inducing sterile lines.

Method used

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Effect test

Embodiment 1

[0035] A method for obtaining male sterile plants of Chinese chives, by irradiating Chinese chive seeds with different doses of cobalt 60 rays, the Chinese chive seeds of this embodiment 1 are shallow dormant Chinese chive inbred seeds, so that the Chinese chive seeds are induced to mutate, In the flowering stage, the sterile plants produced by the inbred line of Chinese chives were found due to the abortion of Chinese chives at the flowering stage.

[0036] The light dormant leek inbred seed of this embodiment 1 refers to that after the inbred seed is planted, when the plant grows to a certain size, after a long period of sunshine, when the temperature drops to about 10 degrees, the leek The plants show growth arrest and begin to go dormant. The inbred seeds with this performance of plants in the field are called shallow dormant chive inbred seeds. Shallow dormancy leeks have two manifestations after dormancy. One is that the plants continue to remain green and will not dry ...

Embodiment 2

[0056] A method for obtaining male sterile plants of Chinese chives, by irradiating Chinese chive seeds with different doses of cobalt-60 rays, the Chinese chive seeds of this embodiment 2 are dormant type chive inbred seeds, so that the Chinese chive seeds are induced to mutate, In the flowering stage, the sterile plants produced by the inbred line of Chinese chives were found due to the abortion of Chinese chives at the flowering stage.

[0057] Specifically include the following steps:

[0058] ) seed selection:

[0059] Select mature and plump dormant chive inbred seeds and place them in polyethylene plastic bags;

[0060]) Radiation Seed:

[0061] The leek seeds of the shallow dormancy type placed in a polyethylene plastic bag are placed in an area of ​​40-120 cm around the radioactive source, and the above-mentioned leek seeds are irradiated with cobalt-60 rays with a radiation dose of 40-60 Gray (Gy). ;

[0062] ) Leek Seedlings:

[0063] The irradiated seeds in st...

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PUM

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Abstract

The invention relates to the technical field of vegetable genetics and breeding, in particular to a method for obtaining male sterile plants of Chinese chives. By using different doses of cobalt 60 rays to irradiate the seeds of Chinese chives, the seeds of Chinese chives are induced to mutate. During the investigation of the flowering period, it was found that sterile plants of Chinese chives were produced in the inbred lines of different strains. The invention breaks the historical process that the sterile leeks are completely formed naturally and discovered by chance in the past, greatly increases the sterile plant rate of the leek strains, and the occurrence probability of the sterile strains in the leek strains reaches 10.5%. The artificial generation of Chinese chive sterile strains is a historic breakthrough in the breeding process of Chinese chives, which makes the formation of male sterile lines of Chinese chives targeted and the formation of new Chinese chive varieties predictable.

Description

technical field [0001] The invention relates to the technical field of vegetable genetics and breeding, in particular to a method for producing male sterile plants in the field through seed radiation induction. Background technique [0002] Leek is a perennial herbaceous perennial plant of the Liliaceae Allium genus. It originated in China and has a cultivation history of more than 2,000 years. It is cultivated everywhere. [0003] There are abundant local varieties of leeks and newly bred varieties in China. If male sterile plants can be found among leek varieties, their clones can be established by tissue culture and rapid propagation technology, and then field ramets can be used for propagation and tissue culture and rapid propagation. The method to expand the sterile line population, and leeks can grow for 20 to 30 years under good cultivation conditions, so it is of practical significance to use male sterile line clones to produce a generation of hybrids. [0004] Plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06A01H1/02
CPCA01H1/02A01H1/06
Inventor 张桂海王明耀王学颖黄志辉田庆武王长浩张钊王铁军王学众马德明张义岭
Owner 廊坊市农林科学院
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