Image collection method and device, microscope system and computer readable storage medium

Abstract

The invention provides an image collection method and device, a microscope system and a computer readable storage medium, the method is applied to the microscope system, the method comprises the following steps: when a to-be-tested sample is a preset sample type, an objective lens is controlled to acquire a first image of the to-be-tested sample on a fitting focal plane, and the first image comprises a target main body; controlling the objective lens to acquire a second image of the to-be-measured sample on each of a plurality of mutually parallel preset planes, wherein the plurality of presetplanes are intersected with the fitting focal plane; and carrying out image fusion on the plurality of second images to obtain a target image, wherein the target image comprises the target main body.According to the method, the second images are collected on the multiple preset planes intersecting with the fitting focal plane, the multiple second images are fused to obtain the target image, thetarget image obtained after fusion of the multiple second images serves as the microscopic examination image, the quality of the microscopic examination image can be improved, the microscopic examination efficiency is improved, and the missing detection probability of the target body is reduced.

Description

technical field [0001] The present application relates to the technical field of sample identification and image processing, in particular to an image acquisition method and device, a microscope system, and a computer-readable storage medium. Background technique [0002] In the process of disease diagnosis and scientific research, it is often necessary to sample and make films of the specimens to be inspected, and use the method of microscopic examination to observe, analyze and judge the sample images under a high-power or low-power microscope, and provide samples for identification. Microscopic examination results. Human excrement, secretions, exfoliated cells or human tissues, animal tissues, and even plant cells can all be used as objects for microscopic examination. [0003] However, due to the uneven processing precision of the sample container, the tilting of the counting plate and blood smear, and the shaking during the mechanical transmission process, the target s...

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Problems solved by technology

[0003] However, due to the uneven processing precision of the sample container, the tilting of the counting plate and blood smear, and the shaking during the mechanical transmission process, the target subjects in all viewing areas may not be on the same preset plane. By adjusting the stage or objective lens It is very difficult to compensate for the slight error of the sample container by the height of the sample, so the quality of the collected microscopic image is poor, and the probability of missing the target is high

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