Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for rapidly obtaining DNA condensate

A condensate, rapid technology, applied in the field of rapid acquisition of DNA condensate, can solve the problem that synergy has not been studied in depth

Active Publication Date: 2020-04-07
WENZHOU UNIVERSITY
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

and Mg 2+ The effect of drugs such as ethanol on DNA condensation has been widely revealed, but the synergistic effect of ethanol and pH on DNA condensation caused by magnesium ions has not been studied in depth

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly obtaining DNA condensate
  • Method for rapidly obtaining DNA condensate
  • Method for rapidly obtaining DNA condensate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0027] The following is the use of three experimental methods—atomic force microscopy (AFM), single-molecule magnetic tweezers (MT) and dynamic light scattering (DLS) techniques to analyze DNA molecules at different pH values, different volume fractions of ethanol, 3mM Mg 2+ Experimental studies were carried out in solution. The changes of DNA aggregation morphology, critical cohesion and electrophoretic mobility were studied by AFM, MT and DLS respectively.

[0028] 1. Experimental process

[0029] 1. Materials

[0030] Ethanol was purchased from Jingke Hongda Company (Shanghai, China), magnesium chloride and other chemical reagents were purchased from Sigma-Aldrich Company, and 5 mmol·L －1 Dilute in Tris buffer. Ultrapure water was deionized and purified thr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for rapidly obtaining a DNA aggregate, according to the invention, a magnesium salt and DNA to-be-aggregated are added to a mixed solution containing water and ethanol, and the mixed solution containing water and ethanol is acidic or an acid reagent is added to make the mixed solution containing water and ethanol become acidic. The buffer solutions with different PH values are used for researching the influence of the combined action of the PH values, the ethanol concentration and the magnesium ions on DNA agglomeration, the PH is lower, the ethanol concentration is higher, the DNA condensation effect is larger, the DNA size is shorter, the time is obviously shortened, and especially under the system that the PH is smaller than or equal to 6, the influence of the ethanol concentration on DNA condensation is obviously increased.

Description

technical field [0001] The invention relates to a method for rapidly obtaining DNA condensates. Background technique [0002] DNA is the carrier of genetic information. The highly condensed and orderly arranged DNA structure maintains the stability of the genome and enables the successful completion of replication. Gene therapy is to introduce human healthy genes or genes with therapeutic effects into disease-causing cells of the human body through an appropriate carrier medium, so as to correct the disease-causing genes and achieve the purpose of treating diseases. With the rise of gene therapy research, there has been renewed interest in the study of DNA condensation, as DNA condensation provides an important tool in gene delivery in gene therapy. DNA condensation is a process in which stretched DNA molecules collapse to form a compact and orderly structure, generally containing one or several molecules. Due to the formation of a circular structure, the DNA size is signif...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 王艳伟戴庭辉罗胜吕方怡许诗雨陈浩迪
Owner WENZHOU UNIVERSITY