The application of tetrahydropyrimidine in regulating the strength of dna condensation
A kind of tetrahydropyrimidine, strong and weak technology, applied in the field of gene therapy
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Embodiment 1
[0024] Example 1 Influence of ectoine on DNA condensation in monovalent counter ion solution:
[0025] Add DNA to multiple sets of mixed solutions (with different concentrations of sodium chloride and different concentrations of ectoine), and measure after standing at room temperature for 5 minutes. During the measurement, a DNA solution in a volume of 1 mL was used, and the sample temperature was kept at 25°C.
[0026] Under the conditions of different ectoine concentrations, the relationship between the electrophoretic mobility of DNA in solution and the concentration of sodium ions and ectoine concentrations is as follows figure 1 shown. exist figure 1 In (a), the electrophoretic mobility (EM) of DNA is plotted against the concentration of sodium ions. As the concentration of sodium ions in the solution increases, we can see that the electrophoretic mobility of the DNA complex increases gradually as expected. When no ectoine was added, the electrophoretic mobility incre...
Embodiment 2
[0029] Example 2 Influence of ectoine on DNA condensation in divalent counter ion solution:
[0030] DNA was added to multiple sets of mixed solutions (with different concentrations of magnesium chloride and different concentrations of ectoine), and the electrophoretic mobility was tested after standing at room temperature for 5 minutes. During the measurement, a DNA solution in a volume of 1 mL was used, and the sample temperature was kept at 25°C.
[0031] Under the conditions of different ectoine concentrations, the relationship between the electrophoretic mobility of DNA in the solution and the concentration of magnesium ions and the concentration of ectoine is as follows: image 3 shown. exist image 3 In (a), the electrophoretic mobility (EM) of DNA versus the concentration of magnesium ions was plotted. Similar to the situation of the monovalent ion solution environment in Example 1, we can see that the electrophoretic mobility of DNA increases with the Mg in the solut...
Embodiment 3
[0033] Example 3 Influence of ectoine on DNA condensation in trivalent counter ion solution:
[0034] DNA was added to multiple mixed solutions (different concentrations of cobalt trichlorohexammine and different concentrations of ectoine), and the electrophoretic mobility was tested after standing at room temperature for 5 minutes. During the measurement, a DNA solution in a volume of 1 mL was used, and the sample temperature was kept at 25°C.
[0035] Under the conditions of different ectoine concentrations, the electrophoretic mobility of DNA in solution is related to [Co(NH 3 ) 6 ] 3+ The relationship between concentration and ectoine concentration is as Figure 5 shown. exist Figure 5 In (a), the electrophoretic mobility (EM) of DNA is plotted against [Co(NH 3 ) 6 ] 3+ concentration relationship curve. With the solution [Co(NH 3 ) 6 ] 3+ As the ion concentration increases, we can see an increase in the electrophoretic mobility of the DNA complex. For example...
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