A kind of high temperature resistant streptomyces, microbial soil reducing agent and preparation method and application thereof
A technology of microorganism and reducing agent, applied in the field of microbial soil reducing agent and its preparation, high temperature resistant Streptomyces, can solve the problems of long time, residue, soil pH imbalance and so on
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[0045] The present invention provides the preparation method of the microbial soil reducing agent described in the above-mentioned technical scheme, is characterized in that, comprises the following steps: (1) Streptomyces vulgaris thermo, Streptomyces grisea and Bacillus subtilis are respectively inoculated on the activation medium Cultivate; (2) the activated Streptomyces vulgaris, Streptomyces cinerea and Bacillus subtilis are respectively inoculated in respective seed culture medium, and cultivated on a shaking table to obtain Streptomyces vulgaris thermos, Streptomyces cinerea and Streptomyces cinerea respectively Seed liquid and Streptomyces grisea seed liquid; (3) The seed liquids of Streptomyces vulgaris, Streptomyces grisea and Bacillus subtilis obtained in step (2) are respectively inoculated in a liquid fermentation medium, and fermented to obtain a fermented (4) mixing the fermented liquids of Streptomyces vulgaris, Streptomyces grisea and Bacillus subtilis obtained...
Embodiment 1
[0064] (1) Strain activation: inoculate Streptomyces vulgaris Sanju-11 and Streptomyces grisea Sanju-09 in Gao's No. 1 medium, and Bacillus subtilis Sanju-07 in beef extract peptone medium to activate.
[0065] (2) Seed liquid preparation: inoculate activated Streptomyces vulgaris, Streptomyces grisea, and Bacillus subtilis with their respective seed liquid culture medium in proportions of 10%, 10%, and 10% by volume respectively in the respective activated In the culture medium, cultivate under 40°C and 150rpm shaking table, cultivate Streptomyces vulgaris and Streptomyces grisea for 48 hours, and Bacillus subtilis for 36 hours to obtain Streptomyces vulgaris thermale, Streptomyces grisea seed solution and Streptomyces grisea respectively. Streptomyces rubrum seed solution.
[0066] (3) Expanded culture by liquid fermentation: inoculate the seed fermentation liquid with an inoculum size of 8% by volume in the liquid fermentation medium.
[0067] Liquid fermentation medium i...
Embodiment 2
[0071] Experimental design: 9 sheds were set up in the experiment, each of which was regarded as 1 treatment, divided into 3 treatments in total, arranged in random blocks, and protected rows were set around.
[0072] Process A blank control;
[0073] Treatment B conventional irrigation high temperature stuffy shed;
[0074] Process C high-temperature stuffy shed+the microbial soil reducing agent prepared by the preparation method of embodiment 1;
[0075] Fertilization management: On July 20, 2017, the stuffy shed treatment was carried out, and treatment A was not treated; treatment B was routinely irrigated and stuffed shed; treatment C was 2L / 666.7m 2The amount of application of the microbial soil reducing agent obtained in Example 1 was diluted 400 times and then sprayed. Cucumbers were planted after 15 days in the stuffy shed. When the cucumbers matured, the incidence and growth conditions were detected. The straw decomposing effect during the stuffy shed period was de...
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