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Alkaline phosphatase detection kit

A technology for detecting kits and phosphatase, which is applied in the field of alkaline phosphatase detection kits, can solve the problems affecting the detection effect and reagent performance degradation, and achieve high detection accuracy and precision, good stability, and guaranteed stability Effect

Pending Publication Date: 2020-04-14
江苏迈源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the detection process, the reagents are in contact with the air for a long time, and the performance of the reagents continues to decline, which affects the detection effect.

Method used

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  • Alkaline phosphatase detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The invention provides an alkaline phosphatase detection kit, the main components of which are as follows:

[0021]

[0022]

[0023] After the reagent is calibrated with the calibrator, the target value is measured at 10 days, 20 days, 30 days, and 60 days respectively, and the calibration will not be repeated during this period.

[0024]

[0025]

[0026] According to the requirements of the instructions, the reagent has a stable period of 30 days after opening the cap, the accuracy is within ±10%, and the precision CV is less than or equal to 4%. The test kit of this embodiment has better accuracy and precision measurement values ​​within 30 days after opening the cover, and the accuracy and precision within 60 days can still meet the measurement requirements. Effectively guarantee the stability of the reagent when it is opened.

Embodiment 2

[0028] The invention provides an alkaline phosphatase detection kit, the main components of which are as follows:

[0029]

[0030] After the reagent is calibrated with the calibrator, the target value is measured at 10 days, 20 days, 30 days, and 60 days respectively, and the calibration will not be repeated during this period.

[0031] opening time After calibration 10 days 20 days 30 days 60 days Accuracy 0.45％ -0.31％ -1.6％ -2.61％ -3.94％ precision 0.8％ 1.6％ 1.9％ 2.4％ 4.79％

[0032] The accuracy and precision of the reagents in this embodiment meet the measurement standards within 60 days.

Embodiment 3

[0034] The invention provides an alkaline phosphatase detection kit, the main components of which are as follows:

[0035]

[0036]

[0037] After the reagent is calibrated with the calibrator, the target value is measured at 10 days, 20 days, 30 days, and 60 days respectively, and the calibration will not be repeated during this period.

[0038] opening time After calibration 10 days 20 days 30 days 60 days Accuracy 1.21％ -1.09％ -1.91％ -2.08％ -3.05％ precision 1.60％ 2.61％ 3.07％ 3.87％ 8.79％

[0039] The accuracy and precision of the reagents in this embodiment meet the measurement standards within 60 days.

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Abstract

The invention provides an alkaline phosphatase detection kit, and belongs to the technical field of medical examination. The kit comprises a reagent 1, a reagent 2, a calibration material and a quality control material. The reagent 1 comprises the following main components: 2-amino-2-methyl-1-propanol (AMP buffer solution with the pH value of 10.0), magnesium acetate, PC300, zinc sulfate and Tween20. The reagent 2 is prepared from the following main components: 2-amino-2-methyl-propanol (AMP buffer solution with the pH value of 10.0), disodium p-nitrophenyl phosphate (4-NPP) and PC300. By adding ethyl acetate, PMX-200 or liquid paraffin into the reagent 1 and the reagent 2, the contact between the reagents and air is effectively reduced, the reaction between the main components in the reagents and oxygen is reduced, and the uncovering stability of the reagents is guaranteed.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to an alkaline phosphatase detection kit. Background technique [0002] Alkaline phosphatase is widely distributed in tissues such as human liver, bone, intestine, kidney and placenta, and is an enzyme excreted from the liver to the bile. The clinical determination of alkaline phosphatase activity is mainly used for the diagnosis of liver and bone diseases. Increased: seen in obstructive jaundice, liver cirrhosis, liver necrosis (the increase is not obvious in hepatocellular jaundice); primary and secondary liver diseases. Also seen in osteitis deformans, osteoblastic disease, rickets, osteomalacia, thyroid and hyperparathyroidism, renal tubular acidosis, hyperphosphatase. Reduced: common in severe chronic nephritis, celiac disease, anemia, cachexia, hypothyroidism or hypothyroidism in children, malnutrition, cretinism, hypophosphatasia. [0003] At present, the detection of a...

Claims

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Application Information

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IPC IPC(8): C12Q1/42
CPCC12Q1/42G01N2333/916
Inventor 孟广宇吉爽爽范潇
Owner 江苏迈源生物科技有限公司
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