Composite probiotic agent as well as preparation method and application thereof

A technology of compounding probiotics and bacterial liquid, applied in application, animal feed, additional food elements, etc., can solve the problems of shortening the peak period of egg production, decline in egg production rate, and decline in egg quality, so as to improve salpingitis and reduce damage. rate, adaptable effect

Active Publication Date: 2020-04-17
HEBEI KEXING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a compound probiotic agent and its preparation method and application, so as to solve the problem

Method used

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  • Composite probiotic agent as well as preparation method and application thereof
  • Composite probiotic agent as well as preparation method and application thereof
  • Composite probiotic agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Chicken-derived Enterococcus lactic acid AR

[0062] A chicken-derived Enterococcus lactic acid ( Enterococcus lactis ) AR, the bacterium was isolated and screened from the contents of hen oviduct, and was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on September 10, 2019. The preservation address is Beichen West Road 1, Chaoyang District, Beijing No. 3 Courtyard, the preservation number is CGMCC No. 18483, and the Latin name is Enterococcus lactis .

[0063] Its 16SrRNA sequence is as follows:

[0064] CGGGCGGTGTGTAACTGCCCGGTAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCGGCTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGAGAAGCTTTAAGAGATTAGCTTAGCCTCGCGACTTCGCAACTCGTTGTACTTCCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTTGCTAGAGTGCCCAACTGAATGATGGCAACTAACAATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACTTTGCCCCCGAAGGGGAAGCTCTATCTCTAGAGTGGTC...

Embodiment 2

[0068] Example 2 Screening method for chicken-derived Enterococcus lactic acid AR

[0069] This embodiment is a screening method for chicken-derived Enterococcus lactic acid AR involved in Example 1, which includes the following steps in sequence:

[0070] a1) It is taken from the oviduct of a hen in a healthy egg-laying period under aseptic conditions, and sealed and preserved under aseptic conditions after taking it out;

[0071] Get the content in the hen's oviduct under aseptic conditions and place it in a sterile anaerobic incubator for standby;

[0072] a2) Inoculate the content on tryptone soy agar medium (TSA medium);

[0073] a3) Place the inoculated TSA medium in an anaerobic incubator at 37°C for static culture for 48 hours to obtain culture E; the anaerobic environment is composed of H 2 , CO 2 and N 2 composition;

[0074] a4) Pick a single colony F from the culture E and inoculate it on the MRS agar medium, and place it in a common bacterial incubator at 37°C ...

Embodiment 3

[0077] Example 3 Bacteriological characteristics of chicken-derived Enterococcus lactic acid AR

[0078] 1. Basic features

[0079] This embodiment is the bacteriological basic characteristics of the chicken source Enterococcus lactic acid AR cultivated in Example 2, and its basic characteristics are shown in the following table:

[0080] Table 1 Basic characteristics of Enterococcus lactis AR from chicken

[0081]

[0082] 2. Biochemical characteristics

[0083] The chicken-derived Enterococcus lactic acid AR of the present invention is a facultative anaerobic bacterium with strong acid resistance, high growth rate and fast acid production rate. Good acid production ability, rapid growth under the condition of pH3.0.

[0084] Streak inoculation of Enterococcus lactic acid AR cultured in Example 2 into medium A, TSA medium, BHI medium, NA medium, LB medium, and place the inoculated medium at 37°C Cultivate in the bacterial incubator for 48h, observe the growth state, se...

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Abstract

The invention discloses a composite probiotic agent, belonging to the field of probiotics for veterinary use. The composite probiotic agent contains chicken-derived enterococcus faecium AR with a concentration of no less than 8.0*10<10> CFU/mL, enterococcus faecium S2 with a concentration of no less than 1.0*10<10> CFU/mL and pediococcus acidilactici with a concentration of no less than 1.0*10<10>CFU/mL, and solves the problem of egg quality reduction caused by salpingitis of chicken in an egg laying period. The invention also discloses a preparation method of the composite probiotic agent. The composite probiotic agent is prepared by compounding the following components in parts by volume: 5-8 parts of bacterial liquid of a chicken-derived enterococcus faecium AR fermentation product, 1-3 parts of bacterial liquid of an enterococcus faecium S2 fermentation product and 1-2 parts of bacterial liquid of a pediococcus acidilactici fermentation product. The invention also provides the application of the composite probiotic agent in improvement of chicken salpingitis. The composite probiotic agent disclosed by the invention can improve bacterial salpingitis of laying fowls, improve eggshell quality and increase egg laying rate, and is used for feeding hens so as to achieve the purposes of preventing and treating diseases.

Description

technical field [0001] The invention belongs to the field of veterinary probiotics, and in particular relates to a compound probiotic agent and its preparation method and application. Background technique [0002] In recent years, due to consumers' increasing attention to food safety, the country's supervision of veterinary antibiotics has become more stringent, and pharmaceutical feed additives will all be withdrawn in 2020. The Ministry of Agriculture and Rural Affairs issued a notice on the pilot work of reducing the use of veterinary antimicrobials from 2018 to 2021, and clarified the timetable for the restriction and prohibition of antibiotics at the breeding end. In the face of the "restricted antibiotics" and "banned antibiotics" policies issued by the state, there is a great market demand for the development of products that replace some of the functions of antibiotics, especially the microecological preparations based on probiotics and their metabolites have a good ...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K20/195
CPCA23K10/18A23K20/195
Inventor 杨锴陈淑芳牛志勇石虎
Owner HEBEI KEXING PHARMA
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